sigV
168
[wiki|RNA polymerase] ECF-type [wiki|sigma factor] SigV, required for resistance to lytic enzymes
locus
BSU_27120
Molecular weight
19.57 kDa
pI
9.04
function
resistance to lytic enzymes
product
[wiki|RNA polymerase] ECF-type [wiki|sigma factor] SigV
essential
no
synonyms
sigV
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1595 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,769,850 → 2,770,350
Phenotypes of a mutant
increased sensitivity to lysozyme, this can be suppressed by overexpression of'' [gene|E97C7A219DB059BB634E893CC355A28324C87ADB|oatA]'' [Pubmed|21856855]
The protein
Protein family
[wiki|Sigma-70 factor family] (according to UniProt)
[wiki|ECF subfamily] (according to UniProt)
Structure
[PDB|5WUQ] (the [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]-[protein|4E720917045032E8B45CB8AF6E5C13AE0E48EE33|rsiW] complex, 28% identity for [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]) [pubmed|28319136]
[AF|O05404]
Effectors of protein activity
activity is controlled by the interaction with [protein|ED1DDA187692683D9862EC0E858B80CD56C48045|rsiV], proteolytic degradation of [protein|ED1DDA187692683D9862EC0E858B80CD56C48045|rsiV] releases active [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV] [Pubmed|23687273]
Additional information
Expression of the [wiki|SigV regulon] in increased in ''[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]'' mutants [Pubmed|22362028]
Expression and Regulation
Operons
genes
[gene|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]-[gene|ED1DDA187692683D9862EC0E858B80CD56C48045|rsiV]-[gene|E97C7A219DB059BB634E893CC355A28324C87ADB|oatA]-[gene|FC37C7A0C0D9F50CA1D44B8D939CF06E210D4B38|yrhK]
description
[Pubmed|21856855]
regulation
induced by lysozyme ([protein|search|SigV]) [Pubmed|21856855]
sigma factors
[protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]: sigma factor, [Pubmed|21856855], in [regulon|protein:D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV regulon]
additional information
A [protein|search|ncRNA] ([wiki|RnaC]) is encoded between '[protein|search|yrhK]' and '[protein|search|yrhJ]' [PubMed|25790031]
additional information
activation occurs heterogeneously in individual cells during lysozyme stress [pubmed|34286912]
Biological materials
Mutant
MGNA-A144 (sigV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/144 NBRP B. subtilis, Japan]
1A907 ( ''sigV''::''kan''), [Pubmed|15838020], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A907&Search=1A907 BGSC]
BKE27120 (Δ[gene|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCAATAAAGGGCTCCT, downstream forward: _UP4_TTGACGAAGGAGGATCTTTC
BKK27120 (Δ[gene|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCAATAAAGGGCTCCT, downstream forward: _UP4_TTGACGAAGGAGGATCTTTC
labs
[wiki|Thomas Wiegert], University of Bayreuth, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/Thomas Wiegert-Dateien/Thomas Wiegert.html Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-18 18:28:41
Author of last update: Jstuelk