safA
168

morphogenetic protein associated with SpoVID, major organizer of the inner spore coat

Locus

BSU_27840

Molecular weight

43.07 kDa

Isoelectric point

5.75

Protein length

387 aaSequenceBlast

Gene length

1164 bpSequenceBlast

Function

spore coat formation

Product

morphogenetic protein

Essential

Synonyms

safA, yrbA

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1388 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

2,844,675 → 2,845,838

Phenotypes of a mutant

mis-assembly of the inner spore coat PubMed

The protein

Domains

contains a N-acetylglucosamine-polymer-binding LysM domain at the N-terminus PubMed

LysM domain (aa 2-47) (according to UniProt)

Structure

O32062 (AlphaFold)

Localization

inner spore coat PubMed

cortex-coat interface PubMed

Expression and Regulation

Operons

Genes

safA-coxA

Description

PubMed

Regulation

expressed early during sporulation in the mother cell (SigE) PubMed

Sigma factors

SigE: sigma factor, PubMed, in sigE regulon

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Biological materials

Mutant

MGNA-A011 (yrbA::erm), available at the NBRP B. subtilis, Japan

1S126 ( safA::erm), PubMed, available at BGSC

1S117 ( safA::spec), PubMed, available at BGSC

BKE27840 (ΔsafA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACGTTTTCCCCTCCTATG, downstream forward: _UP4_TGATCGTTCGGAACGATGTA

BKK27840 (ΔsafA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACGTTTTCCCCTCCTATG, downstream forward: _UP4_TGATCGTTCGGAACGATGTA

Labs working on this gene/protein

Adriano Henriques, Lisbon, Portugal homepage

Charles Moran, Emory University, NC, USA homepage

References

Reviews

Original Publications

Page visits: 4761

Time of last update: 2025-03-15 11:13:18

Author of last update: Jstuelk

safA 168

safA
168