thrZ
168
threonyl-tRNA synthetase (minor)
locus
BSU_37560
Molecular weight
73.20 kDa
pI
5.75
function
translation
product
threonyl-tRNA synthetase (minor)
essential
no
ec
6.1.1.3
synonyms
thrZ, thrS2
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0441 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,854,256 → 3,856,172
The protein
Catalyzed reaction/ biological activity
ATP + L-threonine + tRNAThr --> AMP + diphosphate + H+ + L-threonyl-tRNAThr (according to UniProt)
Protein family
[wiki|Class-II aminoacyl-tRNA synthetase family] (according to UniProt)
[wiki|Domains]
[wiki|TGS domain] (aa 1-64) (according to UniProt)
Structure
[PDB|1TJE] (from ''Escherichia coli'', 43% identity, 65% similarity) [Pubmed|15525511]
[AF|P18256]
Paralogous protein(s)
[protein|3E1131CFA3EDEB3865638F09BE2F6B6ED2570BE2|thrS], one of the two proteins has to be present for viability [Pubmed|17114254]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]
Expression and Regulation
Operons
genes
[gene|CA74CB721842927F67FBC3B5C27D2EE46B30CE1C|thrZ]-[gene|4A3CA47533B217F595E7B600923DA31228B94DA8|ywhA]
description
[Pubmed|1379177]
regulation
induced by threonine limitation ([wiki|T-box]) [Pubmed|19258532]
the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
T-box: RNA switch, in [regulon|other_regulator:T-box|T-box]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1379177], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE37560 (Δ[gene|CA74CB721842927F67FBC3B5C27D2EE46B30CE1C|thrZ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTAGAACACTCCTTAT, downstream forward: _UP4_TAGGATAAAAGCTGTGAATT
BKK37560 (Δ[gene|CA74CB721842927F67FBC3B5C27D2EE46B30CE1C|thrZ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTAGAACACTCCTTAT, downstream forward: _UP4_TAGGATAAAAGCTGTGAATT
References
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Time of last update: 2025-04-07 06:52:16
Author of last update: Melvin.boenninger