cshB

cshB
168

DEAD-box RNA helicase, important for adaptation to low temperatures

Locus
BSU_25140
Molecular weight
49.86 kDa
Isoelectric point
9.89
Protein length
438 aaSequenceBlast
Gene length
1317 bpSequenceBlast
Function
RNA helicase
Product
DEAD-box RNA helicase
Essential
no
Synonyms
cshB, yqfR
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0513 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,594,184 → 2,595,500
Phenotypes of a mutant
poor growth at low temperatures (16 to 20°C) PubMed
The protein
Catalyzed reaction/ biological activity
RNA helicase
required for ribosome assembly PubMed
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
Protein family
helicase family (according to UniProt)
DEAD-box RNA helicases (according to UniProt)
Domains
Helicase ATP-binding domain (aa 35-208) (according to UniProt)
Helicase C-terminal domain (aa 235-385) (according to UniProt)
Structure
5IVL (PDB) (CshA, 80% coverage, 40% identity) PubMed
P54475 (AlphaFold)
Paralogous protein(s)
CshA, DeaD, YfmL
Localization
cytoplasma, colocalizes with the ribosomes PubMed
Expression and Regulation
Operons
Genes
cshB-nfo
Description
PubMed
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cshB-nfo

2025-03-29 11:03:15

Jstuelk

83

3ec11e2631359491cb946c9f57cb14b251d0a506

AAD3EE963744E2A28565ADF40A6EB9DBA6689378

Biological materials
Mutant
MGNA-C489 (cshB::erm), available at the NBRP B. subtilis, Japan
GP1051 (ΔcshB::cat), available in Jörg Stülke's lab
BKE25140 (ΔcshB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTCCCAGCTCCTTAA,  downstream forward: _UP4_AAGAAAAGAAAGTAGGGGAA
BKK25140 (ΔcshB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTCCCAGCTCCTTAA,  downstream forward: _UP4_AAGAAAAGAAAGTAGGGGAA
Expression vectors
for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1064 (spc), available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed
FLAG-tag construct
GP1011 (spc, based on pGP1331), available in Jörg Stülke's lab
Labs working on this gene/protein
Mohamed Marahiel, Marburg University, Germany homepage
References
Reviews
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Original Publications
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C83BD80DE0FD187D07B914F64696438ED5130107

Page visits: 5343

Time of last update: 2025-04-05 01:17:39

Author of last update: Jstuelk