malS

malS
168

malate dehydrogenase (decarboxylating), forms a transhydrogenation cycle with [protein|search|YtsJ ]for balancing of NADPH

locus
BSU_29880
Molecular weight
61.98 kDa
pI
4.89
Protein length
566 aaSequenceBlast
Gene length
1701 bpSequenceBlast
function
malate utilization
product
malate dehydrogenase (decarboxylating)
essential
no
ec
1.1.1.38
synonyms
malS
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0281 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,056,849 → 3,058,549
The protein
Catalyzed reaction/ biological activity
(S)-malate + NAD+ --> CO2 + NADH + pyruvate (according to UniProt)
H+ + oxaloacetate --> CO2 + pyruvate (according to UniProt)
Protein family
[wiki|malic enzymes family] (according to UniProt)
Structure
[PDB|1LLQ] (from ''Ascaris suum'', 40% identity) [Pubmed|12033925]
[AF|O34389]
Paralogous protein(s)
[protein|4F30A4412768E6313F57BD56C4EF5079F500E0C4|maeA], [protein|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|ytsJ], [protein|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]
Expression and Regulation
Operons
genes
[gene|53259B300509E6B6DAF3ED106D1DDEDC99B5532B|ytnP]-[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]
description
[Pubmed|22383849]
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[gene|53259B300509E6B6DAF3ED106D1DDEDC99B5532B|ytnP]→[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]

2025-03-09 12:39:38

Jstuelk

77

75ba9e5ed484435744ff752a0d52b953d2db660b

3FA5F12E33DF792D63A9B9469DEF97DD8C9A3A48

genes
[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]
description
[Pubmed|9387221]
Open in new tab

[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]

2025-03-10 23:26:04

ghost

69

cb04c3712dda5212739244b42ade7f7cc6c77b87

E709C34D26169D4727A83EC08555F5ED876D4156

Biological materials
Mutant
GP1142 (Δ[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]::spc) available in [wiki|Jörg Stülke]'s lab
BKE29880 (Δ[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACCCTGCCTCTTCCTTTCT,  downstream forward: _UP4_TAATAAAAAAGAGCCGCTGC
BKK29880 (Δ[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACCCTGCCTCTTCCTTTCT,  downstream forward: _UP4_TAATAAAAAAGAGCCGCTGC
References
9387221,16788182,12949160,23136871,22740702,12033925,32759333

BBBCD5F56779895189E21076AF165B901F654534

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Time of last update: 2025-04-03 19:37:07

Author of last update: Jstuelk