mnmM
168
SAM-dependent class I 5-aminomethyl-2-thiouridine methyltransferase
locus
BSU_30490
Molecular weight
21.14 kDa
pI
5.56
function
tRNA modification at U34
product
SAM-dependent class I 5-aminomethyl-2-thiouridine methyltransferase
essential
no
synonyms
ytqB, MnmM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0275 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,120,964 → 3,121,548
The protein
Catalyzed reaction/ biological activity
modification of tRNA at U34, conversion of 5-aminomethyl-(2-thio)uridine to 5-methylaminomethyl-(2-thio)uridine [pubmed|36762482]
Protein family
[wiki|Methyltransferase superfamily] (according to UniProt)
[wiki|Cofactors]
SAM [Pubmed|24637210]
Structure
[PDB|4PON], apoenzyme [Pubmed|24637210]
[PDB|4POO], complex with SAM [Pubmed|24637210]
[PDB|8H1B], [protein|B7B3DF7FFA4144CBB12A72996C73CFBD7B6DD2D8|mnmM] from S. aureus complexed with SAM and tRNA anti-codon stem loop
[AF|O34614]
Expression and Regulation
Operons
genes
[gene|AB9762F1D388E27039FEFE946D8BE527CEF13F09|mnmL]-[gene|B7B3DF7FFA4144CBB12A72996C73CFBD7B6DD2D8|mnmM]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A165 (ytqB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/165 NBRP B. subtilis, Japan]
BKE30490 (Δ[gene|B7B3DF7FFA4144CBB12A72996C73CFBD7B6DD2D8|mnmM]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTAAGGAAGAATTTTCTTCA, downstream forward: _UP4_GTCGCCATCGAAAAAAAAGC
BKK30490 (Δ[gene|B7B3DF7FFA4144CBB12A72996C73CFBD7B6DD2D8|mnmM]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTAAGGAAGAATTTTCTTCA, downstream forward: _UP4_GTCGCCATCGAAAAAAAAGC
References
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Time of last update: 2025-02-03 06:42:03
Author of last update: Jstuelk