ytdI
168
inorganic polyphosphate/ATP-NAD kinase
locus
BSU_29540
Molecular weight
30.10 kDa
pI
6.49
function
generation of NADP from NAD
product
inorganic polyphosphate/ATP-NAD kinase
essential
no
ec
2.7.1.23
synonyms
ytdI, ppnKB, nadK2
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0061 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,021,233 → 3,022,036
The protein
Catalyzed reaction/ biological activity
ATP + NAD+ --> ADP + H+ + NADP+ (according to UniProt)
Protein family
NAD kinase family (with [protein|2A2640A0501ECEA44A3C0C4842FAC053FB422A1F|nadF], according to UniProt)
Structure
[PDB|1YT5] (from ''Thermotoga maritima'')\t[Pubmed|16511117]
[AF|O34934]
Paralogous protein(s)
[protein|2A2640A0501ECEA44A3C0C4842FAC053FB422A1F|nadF]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|ytdI]
description
[Pubmed|21815947]
additional information
half-life of the mRNA: 8.4 min, in a [gene|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS] mutant 15 min, in a [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|rny] mutant 24 min [PubMed|25643072]
Other regulations
[protein|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS]: translation inhibition, inhibition of translation and initiation of RNA degradation by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|rny] and [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|rnc] [Pubmed|25643072]
translation is inhibited by binding of the [protein|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS] RNA to the mRNA [Pubmed|25643072]
Biological materials
Mutant
MGNA-A109 (ytdI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/109 NBRP B. subtilis, Japan]
BKE29540 (Δ[gene|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|ytdI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCCCATTTCCCCTTTG, downstream forward: _UP4_TAGAAAATAAAAGGACAGGC
BKK29540 (Δ[gene|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|ytdI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCCCATTTCCCCTTTG, downstream forward: _UP4_TAGAAAATAAAAGGACAGGC
References
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Time of last update: 2025-01-23 01:39:43
Author of last update: Jstuelk