efeO
168
lipoprotein, elemental iron uptake system (binding protein), high affinity uptake of ferric iron (Fe(III))
locus
BSU_38270
Molecular weight
42.63 kDa
pI
4.71
function
elemental iron uptake
product
elemental iron uptake system (binding protein)
essential
no
synonyms
efeO, ywbM, efeM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2822 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,927,951 → 3,929,108
Phenotypes of a mutant
no growth with ferric iron (Fe3+) as the sole source of iron [pubmed|23764491]
The protein
Catalyzed reaction/ biological activity
binds ferric iron (Fe(III)) with strong selectivity [Pubmed|23764491]
Protein family
EfeM/EfeO family (single member, according to UniProt)
Structure
[PDB|3AT7] (from ''Sphingomonas sp.'', 40% identity) [Pubmed|21238429]
[AF|P39596]
[wiki|Localization]
membrane [Pubmed|18763711]
extracytosolic lipoprotein [Pubmed|23764491]
Expression and Regulation
Operons
genes
[gene|3BED98E311EF380AE77327C7F0A83254805089C0|efeU]-[gene|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO]-[gene|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB]
description
[Pubmed|9353933]
regulation
immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [Pubmed|29133393,12354229]
regulatory mechanism
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23764491], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B227 (ywbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1226 NBRP B. subtilis, Japan]
BKE38270 (Δ[gene|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGATACAGCGATTTTTGTGA, downstream forward: _UP4_CTATAAAAGGAGTTTTGTAA
BKK38270 (Δ[gene|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGATACAGCGATTTTTGTGA, downstream forward: _UP4_CTATAAAAGGAGTTTTGTAA
References
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Time of last update: 2025-03-27 08:41:59
Author of last update: Robert.warneke