acoA

acoA
168

acetoin dehydrogenase E1 component (TPP-dependent alpha subunit)

Locus
BSU_08060
Molecular weight
35.91 kDa
Isoelectric point
4.91
Protein length
333 aaSequenceBlast
Gene length
1002 bpSequenceBlast
Function
acetoin utilization
Product
acetoin dehydrogenase E1 component (TPP-dependent alpha subunit)
Essential
no
Synonyms
acoA, yfjK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1071 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
879,002 → 880,003
The protein
Cofactors
TPP PubMed
Structure
6CFO (PDB) (human PDH, E1, 38% identity) PubMed
O31404 (AlphaFold)
Paralogous protein(s)
PdhA, BkdAA
Localization
cytoplasm (homogeneous) PubMed
Expression and Regulation
Operons
Genes
acoA-acoB-acoC-acoL
Description
PubMed
Regulation
induced by acetoin (AcoR) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
AcoR: activation, (interaction with SigL-containing RNA polymerase) [ PubMed|11274109], in acoR regulon
Fnr: indirect positive regulation, in fnr regulon
Sigma factors
SigL: sigma factor, PubMed, in sigL regulon
Additional information
the mRNA is substantially stabilized upon depletion of RNase Y PubMed
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acoA→acoL

2025-03-14 02:09:32

Bzhu

121

13d8d82e494fdc730b04ec27f52013adc2c6a3b0

0F3039221AB7F088A4D6093D7D4525D99014DE74

Additional information
the mRNA is very stable (> 15 min) PubMed
Biological materials
Mutant
MGNA-C280 (acoA::erm), available at the NBRP B. subtilis, Japan
BKE08060 (ΔacoA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTGTGCGCCTCCTTCT,  downstream forward: _UP4_TATGAAAAAGGAGGAATGTA
BKK08060 (ΔacoA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTGTGCGCCTCCTTCT,  downstream forward: _UP4_TATGAAAAAGGAGGAATGTA
References
Reviews
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Original Publications
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B0A333BDFAE816B48B856A1DD91B73C41584A8E4

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Time of last update: 2025-03-15 12:02:17

Author of last update: Jstuelk