mreB
168
[wiki|cell shape]-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for [protein|search|LytE ]activity, part of the [protein|search|Rod complex] for lateral [wiki|cell wall synthesis] and control of cell diameter
locus
BSU_28030
Molecular weight
35.79 kDa
pI
4.9
function
[wiki|cell shape] determination
product
[wiki|cell shape]-determining protein
essential
yes
synonyms
mreB, divIVB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1077 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,860,735 2,861,748
Phenotypes of a mutant
essential [Pubmed|12682299]
the mutation can be suppressed by addition of 5 mM magnesium to the growth medium, this results in inhibition of peptidoglycan hydrolyses such as [protein|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE] [Pubmed|35064120,15752190]
the mutation can be suppressed by inactivation of ''[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA]'', ''[gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]'', ''[gene|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]'' [Pubmed|19192185], or by overexpression of [protein|7150BABA5086D5E2EB8102E4901A216F43576282|glmR] [Pubmed|21320184]
rougher, softer cell surfaces, loss of cell shape, cell lysis [pubmed|35064120]
The protein
Catalyzed reaction/ biological activity
forms straight filaments in a heterologous system [Pubmed|21091501]
polymerizes in the presence of millimolar divalent cations, binds and hydrolyzes GTP and ATP [Pubmed|19117023]
involved in the organization of 29 DNA replication machinery in peripheral helix-like structures [Pubmed|22420858]
required for [protein|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE] activity [Pubmed|23869552]
Protein family
[wiki|FtsA/MreB family] (according to UniProt)
Structure
[PDB|1JCF] (from ''Thermotoga maritima'', 57% identity) [Pubmed|11544518]
[AF|Q01465]
Modification
acetylated at Lys-240, this is important for restricting cell wall growth and cell diameter [Pubmed|27376153]
Paralogous protein(s)
[protein|40829C3632E9DCEB9E1C14A674B4B217F504CFE0|mreBH], [protein|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl]
[wiki|Localization]
during logarithmic growth, [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis [Pubmed|21636744]
forms transverse bands as cells enter the stationary phase [Pubmed|21636744]
forms antiparallel double filaments [Pubmed|24843005]
close to the inner surface of the cytoplasmic membrane [Pubmed|16950129]
reports on helical structures formed by MreB [Pubmed|16950129,20566861] seem to be misinterpretation of data [Pubmed|21636744]
normal localization depends on the presence of glucolipids, [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] forms irregular clusters in an [gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP] mutant [Pubmed|22362028]
during stationary phase: delocalized in the cytoplasm [Pubmed|26091431]
during development of [wiki|genetic competence]: co-localizes with polar clusters of the late competence protein [protein|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA] ([protein|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA] sequesters [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]) [Pubmed|26091431]
MreB patch speed depends on the growth rate, the faster growth, the higher the patch speed [pubmed|28589952]
MreB filament intensity increases with the growth rate and depends on the abundance of lipid II and [protein|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA] due to increased [protein|6071A84EF190C820DB8985C08ABC74F744B793DB|rodZ] phosphorylation by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC] [pubmed|36797487]
Expression and Regulation
Operons
genes
[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
description
[Pubmed|8459776]
regulation
constitutively expressed [Pubmed|23701187]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|26091431], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|26091431], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, promoter p2, within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, promoter p4, upstream of [protein|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC] [Pubmed|8459776], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
genes
[gene|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf]-[gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]-[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
description
[Pubmed|22383849]
regulation
constitutively expressed [Pubmed|23701187]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|11948146,11918817,21564336], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, promoter p2 within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|26091431], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in the labs of [wiki|Jeff Errington] and [wiki|Boris Görke]
Antibody
available in the [wiki|Jeff Errington] and [wiki|Peter Graumann] labs
labs
[wiki|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]
[wiki|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
References
Reviews
Original Publications
Localization
Page visits: 10641
Time of last update: 2024-12-18 05:41:40
Author of last update: Jstuelk