pdhA
168
pyruvate dehydrogenase (E1 alpha subunit), required for Z-ring assembly in a pyruvate-dependent manner
Locus
BSU_14580
Molecular weight
41.39 kDa
Isoelectric point
5.84
Function
links glycolysis and TCA cycle
Product
pyruvate dehydrogenase (E1 alpha subunit)
Essential
no
E.C.
1.2.4.1
Synonyms
pdhA, aceA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1071 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,528,326 1,529,441
Phenotypes of a mutant
The protein
Catalyzed reaction/ biological activity
[dihydrolipoyllysine-residue acetyltransferase]-(R)-N6-lipoyl-L-lysine + H+ + pyruvate --> [dihydrolipoyllysine-residue acetyltransferase]-(R)-N6-(S8-acetyldihydrolipoyl)-L-lysine + CO2 (according to UniProt)
TPP PubMed
Structure
1W88 (PDB) (E1 in complex with subunit binding domain of E2, Geobacillus stearothermophilus) PubMed
Effectors of protein activity
Inhibited thiamine 2-thiothiazolone diphosphate and NADH PubMed
Low sensibility to NADPH
Kinetic information
Michaelis-Menten PubMed
Paralogous protein(s)
colocalizes with the nucleoid (depending on the availability of pyruvate) PubMed
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Description
Regulation
Regulatory mechanism
stringent response: negative regulation, due to presence of guanine at 1 position of the transcript PubMed, in stringent response
Sigma factors
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Biological materials
Mutant
Expression vectors
pGP2823 (for the purification of PdhA-PdhB-His6 in E. coli, in pET28a), available in Jörg Stülke's lab
pGP2900 (for the purification of Strep-PdhA-PdhB in E. coli, in pGP172), available in Jörg Stülke's lab
LacZ fusion
Labs working on this gene/protein
Arthur Aronson, Purdue University, West Lafayette, USA homepage
References
Reviews
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Original Publications
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Time of last update: 2025-03-16 19:11:45
Author of last update: Jstuelk