pdhA
168
pyruvate dehydrogenase (E1 alpha subunit), required for Z-ring assembly in a pyruvate-dependent manner
locus
BSU_14580
Molecular weight
41.39 kDa
pI
5.84
function
links glycolysis and TCA cycle
product
pyruvate dehydrogenase (E1 alpha subunit)
essential
no
ec
1.2.4.1
synonyms
pdhA, aceA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1071 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,528,326 1,529,441
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
''pdhA'' is essential according to Kobayashi ''et al''. [Pubmed|12682299], non-essential according to [Pubmed|28189581]
the mutant grows slowly but is viable [Pubmed|24825009]
depletion of [gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA] and deletion of [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] have a strong synthetic defect in [wiki|cell division] [Pubmed|24825009]
The protein
Catalyzed reaction/ biological activity
[dihydrolipoyllysine-residue acetyltransferase]-(R)-N6-lipoyl-L-lysine + H+ + pyruvate --> [dihydrolipoyllysine-residue acetyltransferase]-(R)-N6-(S8-acetyldihydrolipoyl)-L-lysine + CO2 (according to UniProt)
[wiki|Cofactors]
TPP [pubmed|35988322]
Structure
[PDB|1W88] (E1 in complex with subunit binding domain of E2, ''Geobacillus stearothermophilus'') [pubmed|15514159]
[AF|P21881]
Effectors of protein activity
Inhibited thiamine 2-thiothiazolone diphosphate and NADH [Pubmed|6414463]
Low sensibility to NADPH
inhibited upon interaction with [protein|5959516D55C9EDBFC46F28C8248D374832895740|pdhI] [pubmed|36815589]
Kinetic information
Michaelis-Menten [Pubmed|6414463]
Paralogous protein(s)
[protein|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA], [protein|B0A333BDFAE816B48B856A1DD91B73C41584A8E4|acoA]
[wiki|Localization]
colocalizes with the nucleoid (depending on the availability of pyruvate) [Pubmed|24825009]
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]-[gene|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|pdhB]-[gene|2F40086E35FA32136B9A89C530A86D714FE9460C|pdhC]-[gene|E9BBAE86DF3E536A987179CC394B472F6F710498|pdhD]
description
[Pubmed|11976308]
regulation
''[protein|search|pdhA]'': expression activated by glucose (1.9-fold) [Pubmed|12850135]
regulatory mechanism
stringent response: negative regulation, due to presence of guanine at 1 position of the transcript [Pubmed|20081037], in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|20081037], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE14580 ([gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTAAGTCACCTCTTCC, downstream forward: _UP4_ACACAGAAGGAGTCGAAGTA
BKK14580 ([gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTAAGTCACCTCTTCC, downstream forward: _UP4_ACACAGAAGGAGTCGAAGTA
Expression vectors
pGP2823 (for the purification of [protein|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]-[protein|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|pdhB]-His6 in E. coli, in pET28a), available in [wiki|Jörg Stülke]'s lab
pGP2900 (for the purification of Strep-[protein|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]-[protein|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|pdhB] in E. coli, in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP721 (in [wiki|pAC5]), available in [wiki|Jörg Stülke]'s lab, pGP186 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]
References
Reviews
Original Publications
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Time of last update: 2025-03-17 11:14:31
Author of last update: Jstuelk