pdhA

pdhA
168

pyruvate dehydrogenase (E1 alpha subunit), required for Z-ring assembly in a pyruvate-dependent manner

Locus
BSU_14580
Molecular weight
41.39 kDa
Isoelectric point
5.84
Protein length
371 aaSequenceBlast
Gene length
1116 bpSequenceBlast
Function
links glycolysis and TCA cycle
Product
pyruvate dehydrogenase (E1 alpha subunit)
Essential
no
E.C.
1.2.4.1
Synonyms
pdhA, aceA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1071 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,528,326 1,529,441
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth PubMed
pdhA is essential according to Kobayashi et al. PubMed, non-essential according to PubMed
the mutant grows slowly but is viable PubMed
depletion of pdhA and deletion of ezrA have a strong synthetic defect in cell division PubMed
The protein
Catalyzed reaction/ biological activity
[dihydrolipoyllysine-residue acetyltransferase]-(R)-N6-lipoyl-L-lysine + H+ + pyruvate --> [dihydrolipoyllysine-residue acetyltransferase]-(R)-N6-(S8-acetyldihydrolipoyl)-L-lysine + CO2 (according to UniProt)
Cofactors
TPP PubMed
Structure
1W88 (PDB) (E1 in complex with subunit binding domain of E2, Geobacillus stearothermophilus) PubMed
P21881 (AlphaFold)
Effectors of protein activity
Inhibited thiamine 2-thiothiazolone diphosphate and NADH PubMed
Low sensibility to NADPH
inhibited upon interaction with PdhI PubMed
Kinetic information
Michaelis-Menten PubMed
Paralogous protein(s)
BkdAA, AcoA
Localization
colocalizes with the nucleoid (depending on the availability of pyruvate) PubMed
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
pdhA-pdhB-pdhC-pdhD
Description
PubMed
Regulation
''pdhA'': expression activated by glucose (1.9-fold) PubMed
Regulatory mechanism
stringent response: negative regulation, due to presence of guanine at 1 position of the transcript PubMed, in stringent response
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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pdhA→pdhD

2025-03-10 01:18:19

ghost

115

797fea7dcbe4317f63eb94d44165272d12ff3ae4

3D5EA61F84C1F6538A394D4F9A00FD4029ED115F

Biological materials
Mutant
BKE14580 (pdhA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACTAAGTCACCTCTTCC, downstream forward: _UP4_ACACAGAAGGAGTCGAAGTA
BKK14580 (pdhA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACTAAGTCACCTCTTCC, downstream forward: _UP4_ACACAGAAGGAGTCGAAGTA
Expression vectors
pGP2823 (for the purification of PdhA-PdhB-His6 in E. coli, in pET28a), available in Jörg Stülke's lab
pGP2900 (for the purification of Strep-PdhA-PdhB in E. coli, in pGP172), available in Jörg Stülke's lab
LacZ fusion
pGP721 (in pAC5), available in Jörg Stülke's lab, pGP186 (in pAC7), available in Jörg Stülke's lab
Labs working on this gene/protein
Arthur Aronson, Purdue University, West Lafayette, USA homepage
References
Reviews
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Original Publications
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953DE0F0B81894ECFF4C0693511AC238BF3D0C0A

Page visits: 8430

Time of last update: 2025-03-16 18:00:46

Author of last update: Jstuelk