hypR
168
[wiki|MarR family|MarR/DUF24 family] transcription regulator, positively controls the nitroreductase gene [gene|9DF5999568760E016ECA988385A1FA61A257D486|hypO] in response to disulfide stress
locus
BSU_40540
Molecular weight
14.59 kDa
pI
8.41
function
control of the nitroreductase gene [gene|9DF5999568760E016ECA988385A1FA61A257D486|hypO] in response to disulfide stress (diamide, NaOCl)
product
[wiki|MarR family|MarR/DUF24 family] transcription regulator HypR
essential
no
synonyms
hypR, yybR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1733 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,167,622 4,167,999
The protein
Protein family
[wiki|MarR family|MarR/DUF24 family]
[wiki|Domains]
5 alpha helices, 2 beta sheets, MarR-fold with wHTH motif, alpha4 major groove recognition helix, beta2 and 3 form the wing; alpha5 dimer interface [Pubmed|22238377]
[wiki|HTH hxlR-type domain] (aa 14-112) (according to UniProt)
Structure
[PDB|4A5N] (reduced HypRC14S dimer) [Pubmed|22238377]
[PDB|4A5M] (oxidized HypR C14-C49' intersubunit disulfide-linked dimer ) [Pubmed|22238377]
[AF|P37486]
Modification
oxidized to Cys14-Cys49' intersubunit disulfides by disulfide stress [Pubmed|22238377]
Cys14 and Cys49' are about 8-9 Angstrm apart in reduced HypR-Dimer, oxidation moves the major groove recognition alpha4 helices of the HypR dimer about 4 Angstroem towards each other that leads to activation of HypR [Pubmed|22238377]
Kinetic information
Cys14 redox sensing Cys, has lower pKa of 6.36 [Pubmed|22238377]
Paralogous protein(s)
[protein|C57DDC9710CB557179B062F9C5D786DB02A3B5FC|ykvN], [protein|D9A187961CFB9496A6712E9E48AAD384357A3E1C|hxlR], [protein|E757C4B329A99E3D0C70C77437DF4DDA300CA5E7|ydzF], [protein|51B1913B59C1876CCED4A88414797B3A22BE4E3C|ydeP], [protein|1E74F0557FF9ECB404B6544831F4E0A162473423|ytcD], [protein|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|yodB]
[wiki|Localization]
cytoplasmic
Expression and Regulation
Operons
genes
[gene|91085DA5E71B88E533D2E1D1BFB53E908116C6AA|hypR]
description
[Pubmed|22238377]
regulation
activated by disulfide stress conditions (diamide, NaOCl) ''in vivo'' and ''in vitro '' (autoregulation) [Pubmed|22238377]
regulatory mechanism
[protein|91085DA5E71B88E533D2E1D1BFB53E908116C6AA|hypR]: activation, (autoregulation)[Pubmed|22238377], in [regulon|protein:91085DA5E71B88E533D2E1D1BFB53E908116C6AA|hypR regulon]
Biological materials
Mutant
MGNA-B836 (yybR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1835 NBRP B. subtilis, Japan]
BKE40540 ([gene|91085DA5E71B88E533D2E1D1BFB53E908116C6AA|hypR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTGACCCTCCAATAG, downstream forward: _UP4_TAGCAGCAAAGGGAACTCCT
BKK40540 ([gene|91085DA5E71B88E533D2E1D1BFB53E908116C6AA|hypR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTGACCCTCCAATAG, downstream forward: _UP4_TAGCAGCAAAGGGAACTCCT
labs
[wiki|Haike Antelmann],University of Greifswald, Germany
Page visits: 3933
Time of last update: 2024-12-18 19:30:44
Author of last update: Melvin.boenninger