thiE
168
thiamine-phosphate pyrophosphorylase
locus
BSU_38290
Molecular weight
23.53 kDa
pI
5.06
function
biosynthesis of thiamine by 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate to yield thiamine phosphate
product
thiamine-phosphate pyrophosphorylase
essential
no
ec
2.5.1.3
synonyms
thiE, ipa-26d, ywbK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0352 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,930,707 3,931,375
The protein
Catalyzed reaction/ biological activity
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H+ --> CO2 + diphosphate + thiamine phosphate (according to UniProt)
2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H+ --> CO2 + diphosphate + thiamine phosphate (according to UniProt)
4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl-5-(2-phosphooxyethyl)-thiazole + H+ --> diphosphate + thiamine phosphate (according to UniProt)
Protein family
thiamine-phosphate synthase family (single member, according to UniProt)
Structure
[PDB|1G6C] [pubmed|11513589]
[AF|P39594]
Paralogous protein(s)
[protein|CA6AAE8AC5B5EF01A911B035F8782579CD876C08|tenI]
Expression and Regulation
Operons
genes
[gene|CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|ywbI]-[gene|9B82059498F72A67282DD54892EF02A305E3C674|thiM]-[gene|8FAEB6342E087BEDC16FDF5585B03B90ACFF8D11|thiE]
description
[Pubmed|9139923]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9139923], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE38290 ([gene|8FAEB6342E087BEDC16FDF5585B03B90ACFF8D11|thiE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATTTCCCGAGAAATAC, downstream forward: _UP4_TAATCGTTCATTTTCCTTCA
BKK38290 ([gene|8FAEB6342E087BEDC16FDF5585B03B90ACFF8D11|thiE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATTTCCCGAGAAATAC, downstream forward: _UP4_TAATCGTTCATTTTCCTTCA
References
Reviews
Original Publications
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Time of last update: 2025-04-07 02:57:07
Author of last update: Melvin.boenninger