minC
168
cell-division inhibitor (septum placement), destabilizes Z ring placement
locus
BSU_28000
Molecular weight
24.85 kDa
pI
6.26
function
septum placement
product
cell-division inhibitor
essential
no
synonyms
minC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0850 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,858,584 2,859,264
Phenotypes of a mutant
a ''[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]'' mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
The protein
Catalyzed reaction/ biological activity
The Min system prevents minicell formation adjacent to recently completed division sites by promoting the disassembly of the cytokinetic ring, thereby ensuring that cell division occurs only once per cell cycle [Pubmed|20352045]
binds the C-terminal domain of [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] to inhibit its polymerization [Pubmed|23577149]
Protein family
minC family (single member, according to UniProt)
Structure
[PDB|4M4I] (N-terminal domain, aa 4 ... 105) [pubmed|39793890]
[PDB|1HF2] (from ''Thermotoga maritima'', 26% identity) [Pubmed|11350934]
[AF|Q01463]
[wiki|Localization]
polar/septal at the cell membrane [Pubmed|20566861]
membrane binding/polar localization depends on the proton motive force [Pubmed|20566861]
Expression and Regulation
Operons
genes
[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
description
[Pubmed|8459776]
regulation
constitutively expressed [Pubmed|23701187]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|26091431], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|26091431], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, promoter p2, within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, promoter p4, upstream of [protein|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC] [Pubmed|8459776], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
genes
[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
description
[Pubmed|8459776]
regulation
constitutively expressed [Pubmed|23701187]
genes
[gene|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf]-[gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]-[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
description
[Pubmed|22383849]
regulation
constitutively expressed [Pubmed|23701187]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|11948146,11918817,21564336], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, promoter p2 within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|26091431], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BSN375 (''[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]''::''aphA3'') (available in [wiki|Leendert Hamoen]'s, [wiki|Sven Halbedel]'s and [wiki|Jörg Stülke]'s labs)
BKE28000 ([gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAATATTCACCTCAACAA, downstream forward: _UP4_ACAAGGCTTGAGGGAGGAAT
BKK28000 ([gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAATATTCACCTCAACAA, downstream forward: _UP4_ACAAGGCTTGAGGGAGGAAT
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
Page visits: 5858
Time of last update: 2025-04-01 13:14:34
Author of last update: Jstuelk