cotE
168
outer spore coat morphogenetic protein, controls the assembly of the outer spore coat layer
locus
BSU_17030
Molecular weight
20.83 kDa
pI
4.14
function
assembly of the outer spore coat
product
spore coat morphogenetic protein
essential
no
synonyms
cotE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5880 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,775,067 1,775,612
Phenotypes of a mutant
mis-assembly of the outer spore coat [Pubmed|22171814]
inactivation of ''[gene|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE]'' reduces sporulation efficiency to 49.1% that of wild type cells [Pubmed|26735940]
The protein
Catalyzed reaction/ biological activity
assembly of the [protein|9C59B51FC19FC83BD14272008BD441833DD1738E|cotC]-[protein|EB690FA075EAC3E298C47AED082B5FC21E8F8F46|cotU] complex [Pubmed|20023017,18065538]
Protein family
cotE family (single member, according to UniProt)
Structure
[AF|P14016]
[wiki|Localization]
encircles the forespore with a biased accumulation on the mother cell-proximal face of the forespore [Pubmed|23267091,22463703]
outer spore coat, localization depends on [protein|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA] [Pubmed|22773792,22171814]
Expression and Regulation
Operons
genes
[gene|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE]
description
[Pubmed|8760914]
regulation
expressed during sporulation in the mother cell ([protein|search|SigE], [protein|search|SigK], [protein|search|GerE]) [Pubmed|1691789,15383836]
regulatory mechanism
[protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: repression, [Pubmed|15383836], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,1691789], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|1691789], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
Biological materials
Mutant
1S105 ( ''cotE''::''cat''), [Pubmed|3139490], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S105&Search=1S105 BGSC]
BKE17030 ([gene|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCGGCATGCCTCCTTG, downstream forward: _UP4_TAAAAAAGGGACTAGGGGAG
BKK17030 ([gene|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCGGCATGCCTCCTTG, downstream forward: _UP4_TAAAAAAGGGACTAGGGGAG
labs
[wiki|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]
[wiki|Adriano Henriques], Lisbon, Portugal [http://www.itqb.unl.pt/research/biology/microbial-development homepage]
References
Reviews
Original Publications
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Time of last update: 2025-03-17 14:32:37
Author of last update: Jstuelk