recO
168
mediator of [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] binding to ssDNA, required for the formation of [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] DNA repair centers, required for efficient survival and replication restart after replication-transcription conflicts
locus
BSU_25280
Molecular weight
29.20 kDa
pI
8.21
function
DNA repair/ recombination
product
mediator of [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] binding to ssDNA
essential
no
synonyms
recO, yqxN, yqfI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1381 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,608,946 2,609,713
Phenotypes of a mutant
strongly reduced survival after mitomycin treatment [pubmed|34339298]
drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) [Pubmed|24285298]
''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]
''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]'' double mutants have a strongly reduced chromosomal transformation rate [Pubmed|23779106,22373918]
''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]
reduced viability of a [gene|48BCA38E609E443406E03A32D2BB8DA2E9915A71|rarA] [gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] double mutant [pubmed|32117122]
suppression of lethality of [gene|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA] inactivation [pubmed|32793628]
The protein
Catalyzed reaction/ biological activity
provides [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] access to ssDNA during chromosomal transformation (together with [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]) [Pubmed|22373918]
catalyzes annealing of [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA] or [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]/[protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB] coated ssDNAs to allow the formation of DNA duplexes with tails during plasmid transformation [Pubmed|22373918]
required for the formation of [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] DNA repair centers (together with [protein|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|recR]) [Pubmed|24891441]
anneals [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]- or [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]-coated complementary strands of transfecting SPP1 phage DNA, yielding tailed SPP1 duplex intermediates [pubmed|31876108]
[protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA], [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] or viral single strand annealing G35P protein, may catalyze the annealing of complete linear phage genomes with redundant regions at the ends of the molecule, alone or with the help of an exonuclease, to produce a circular unit-length duplex viral genome ready to initiate replication [pubmed|31876108]
Protein family
recO family (single member, according to UniProt)
Structure
[PDB|2V1C] (the [protein|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|recR]-[protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] complex from ''Deinococcus radiodurans'', [protein|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|recR]: 52% identity, 78% similarity, [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]: 30%/ 58%) [Pubmed|17581636]
[AF|P42095]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
localizes to the DNA entry pole during transformation [Pubmed|22373918]
Expression and Regulation
Operons
genes
[gene|0E9E9615CE056BEA2B65C494D946EB45F42A3064|cdd]-[gene|CB68B3393CA7862C45C624AF7B1ADD188243A01F|era]-[gene|33FBC31E8C24177137B0BDD8B46C1AFFD7F7B038|yqzL]-[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]
description
[Pubmed|22383849]
expression
expression is increased upon depletion of depletion of tRNA maturation factors [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] or [gene|C40C5D35ED53D343C8200248FCCB010BAB388054|rnz] [pubmed|36840557]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|6330116], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|6330116], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
GP3527 (Δ[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]::kan), available in [wiki|Jörg Stülke]'s lab
MGNA-C494 (yqxN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2492 NBRP B. subtilis, Japan]
1A892 ( ''recO''::''cat''), [Pubmed|10323239], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A892&Search=1A892 BGSC]
BG128 (Δ[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]::cat), available in [wiki|Juan Alonso]'s and [wiki|Jörg Stülke]'s labs [pubmed|9642195]
BP738 ( Δ''recO''::''tet''), (available in [wiki|Fabian Commichau]'s lab)
BP774 ( Δ''recO''::''ermC''), (available in [wiki|Fabian Commichau]'s lab)
BKE25280 (Δ[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGCACCTTCCTCAA, downstream forward: _UP4_TGACATTTGGTCCATCTTTT
BKK25280 (Δ[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGCACCTTCCTCAA, downstream forward: _UP4_TGACATTTGGTCCATCTTTT
References
Reviews
Original Publications
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Time of last update: 2025-04-02 12:57:23
Author of last update: Jstuelk