mdfA
168
metabolic differentiation factor A, sporulation-specific adaptor protein, targets proteins to degradation by [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP] during sporulation
locus
BSU_11410
Molecular weight
29.97 kDa
pI
5.15
function
control of [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]-mediated degradation of metabolic enzymes in the forespore
product
metabolic differentiation factor A, adaptor protein
essential
no
ec
null
synonyms
yjbA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5917 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,217,326 1,218,078
Phenotypes of a mutant
mutant: increased [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]-dependent gene expression during early sporulation [pubmed|40086879]
mutant: reduced sporulation (20 ... 30% of wild type) [pubmed|40086879]
overexpression of [protein|7CFDFB42AF816FF5766086E537472008E6CB7E85|mdfA]: toxic during vegetative growth, this can be suppressed by inactivation of [gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] [pubmed|40086879]
reduced spore resistance to oxidative stress
The protein
Catalyzed reaction/ biological activity
targets [protein|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ] for [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]-dependent degradation in the forespore [pubmed|40086876]
Protein family
UPF0736 family (single member, according to UniProt)
[wiki|Domains]
N-terminal domain: aa 1 ... 135 [pubmed|40086879]
C-terminal domain: aa 143 ... 250 [pubmed|40086879]
Structure
[PDB|8B3S] (the [protein|7CFDFB42AF816FF5766086E537472008E6CB7E85|mdfA]-[protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] complex) [pubmed|40086879]
[AF|O31597]
[wiki|Localization]
peripheral forespore membrane protein [pubmed|40086879]
forespore cytoplasm [pubmed|40086876]
Expression and Regulation
Operons
genes
[gene|7CFDFB42AF816FF5766086E537472008E6CB7E85|mdfA]
description
[pubmed|22383849]
regulation
expressed from 2 hrs after onset of sporulation ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [https://www.biorxiv.org/content/10.1101/2024.03.02.583065v1.full.pdf bioRxiv]
sigma factors
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
Biological materials
Mutant
MGNA-B149 (yjbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1148 NBRP B. subtilis, Japan]
BKE11410 ([gene|7CFDFB42AF816FF5766086E537472008E6CB7E85|mdfA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTAACCCCTCACTTT, downstream forward: _UP4_TGAAAAAAACCGCTCTTTGC
BKK11410 ([gene|7CFDFB42AF816FF5766086E537472008E6CB7E85|mdfA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTAACCCCTCACTTT, downstream forward: _UP4_TGAAAAAAACCGCTCTTTGC
References
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Time of last update: 2025-04-29 22:01:03
Author of last update: Jstuelk