copA
168
copper-exporting ATPase, resistance to copper
locus
BSU_33500
Molecular weight
85.83 kDa
pI
5.48
function
copper export, detoxification
product
copper transporting ATPase
essential
no
synonyms
copA, yvgX
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2608 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,441,121 → 3,443,529
The protein
Catalyzed reaction/ biological activity
ATP + Cu+ + H2O --> ADP + Cu+ + H+ + phosphate (according to UniProt)
Protein family
[wiki|cation transport ATPase (P-type) (TC 3.A.3) family] (according to UniProt)
[wiki|Domains]
two N-terminal soluble domains, CopAa and CopAb, connected by a short linker [pubmed|29146226]
2 HMA domains (aa 6-72, aa 74-140) (according to UniProt)
Structure
[PDB|4BBJ] (the protein from ''Legionella pneumophila'', 45% identity, 79% similarity) [Pubmed|24317491]
[PDB|2RML] ( N-terminal soluble domain) [pubmed|18215122]
[AF|O32220]
Paralogous protein(s)
[protein|017A57F27DD8E515242FB658A61E74C1D58273CC|pfeT], [protein|5BC6B02770E74FF6D453742832574A87BB2369B8|cadA]
[wiki|Localization]
cell membrane (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|6977F18870004AD236539D9409255815E6BE9241|csoR]-[gene|18506FB74F6BB9278F22EB7DCDA3CF72575CC32A|copZ]-[gene|727024F7B1AC19676ED4B516CF46A47B1328310B|copA]
description
[Pubmed|22383849]
genes
[gene|18506FB74F6BB9278F22EB7DCDA3CF72575CC32A|copZ]-[gene|727024F7B1AC19676ED4B516CF46A47B1328310B|copA]
description
[Pubmed|12779235]
regulation
induced by copper ([protein|search|CsoR]) [Pubmed|18048925,12779235]
regulatory mechanism
[protein|6977F18870004AD236539D9409255815E6BE9241|csoR]: repression, [Pubmed|18048925], in [regulon|protein:6977F18870004AD236539D9409255815E6BE9241|csoR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12779235], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A490 (yvgX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/490 NBRP B. subtilis, Japan]
BKE33500 (Δ[gene|727024F7B1AC19676ED4B516CF46A47B1328310B|copA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACATACTCACTCCTTTAT, downstream forward: _UP4_TGAAAAACCGGCTATATGCC
BKK33500 (Δ[gene|727024F7B1AC19676ED4B516CF46A47B1328310B|copA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACATACTCACTCCTTTAT, downstream forward: _UP4_TGAAAAACCGGCTATATGCC
labs
[wiki|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
References
Reviews
Original Publications
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Time of last update: 2025-04-02 11:19:33
Author of last update: Jstuelk