mgsR
168
transcriptional regulator of a subset of the [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB] general stress regulon, required for protection against oxidative stress
locus
BSU_24770
Molecular weight
14.68 kDa
pI
9.28
function
controls a subset of general stress genes
product
transcriptional regulator
essential
no
synonyms
mgsR, yqgZ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1393 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,564,026 2,564,406
The protein
Catalyzed reaction/ biological activity
exerts a positive or negative effect on the expression of 53 or 18 stress genes, respectively [Pubmed|18643936]
Protein family
ArsC family (with [protein|2C6386E9A63F410558D168798D077DF91590F454|spx] and [protein|5278DD377B110CC14E239B9428F1985B472EEDF5|yusI], according to UniProt)
Structure
[PDB|3L78] (from Streptococcus mutans, 42% identity)
[AF|P54503]
Modification
[protein|6A38DAA96F7BBF31FD8A4018A8CA7A72F3C28F79|mgsR] is subject to post-translational redox-sensitive activation by intramolecular disulfide bond formation in response to ethanol stress [Pubmed|22812682]
phosphorylation on R17, R95 by [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB], this tags the protein for degradation by [protein|297F53DAD3351E0C55108DD2C93B78FFB174438C|clpX]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP] [pubmed|32477307,30962353]
Paralogous protein(s)
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]
Expression and Regulation
Operons
genes
[gene|6A38DAA96F7BBF31FD8A4018A8CA7A72F3C28F79|mgsR]
description
[Pubmed|18643936]
regulation
induced by stress ([protein|search|SigB]) [Pubmed|15805528,18643936]
regulatory mechanism
[protein|6A38DAA96F7BBF31FD8A4018A8CA7A72F3C28F79|mgsR]: activation, in [regulon|protein:6A38DAA96F7BBF31FD8A4018A8CA7A72F3C28F79|mgsR regulon]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|18643936], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
additional information
[protein|search|MgsR] is subject to post-translational redox-sensitive activation by intramolecular disulfide bond formation in response to ethanol stress [PubMed|22812682]
additional information
half-life of the protein: 6 minutes [pubmed|32477307]
Biological materials
Mutant
MGNA-C476 (yqgZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2474 NBRP B. subtilis, Japan]
BKE24770 ([gene|6A38DAA96F7BBF31FD8A4018A8CA7A72F3C28F79|mgsR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCGTTTTTTCCTCCTCA, downstream forward: _UP4_TGATGAAAAACCGAAGATCC
BKK24770 ([gene|6A38DAA96F7BBF31FD8A4018A8CA7A72F3C28F79|mgsR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCGTTTTTTCCTCCTCA, downstream forward: _UP4_TGATGAAAAACCGAAGATCC
References
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Time of last update: 2024-12-18 15:45:58
Author of last update: Jstuelk