yfkC
168
mechanosensitive channel, similar to MscS
locus
BSU_07940
Molecular weight
32.05 kDa
pI
5.29
function
resistance to osmotic downshock
product
mechanosensitive channel, similar to MscS
essential
no
synonyms
yfkC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0668 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
867,164 868,006
Phenotypes of a mutant
a [protein|5ACE71EBBDCEB0C534A91DE303FD8069BEFDD6A1|yfkC]-R42W gain of function mutation facilitates the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate [pubmed|33481774]
The protein
Catalyzed reaction/ biological activity
the [protein|5ACE71EBBDCEB0C534A91DE303FD8069BEFDD6A1|yfkC]-R42W gain of function variant converts [protein|5ACE71EBBDCEB0C534A91DE303FD8069BEFDD6A1|yfkC] to a glutamate exporter (in conjunction with [gene|FC05D5FC95CEDC4B4A04040C86170D185528B9FF|plsC], [gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA], or [gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC] mutations that result in reduced phospholipid bisynthesis) [pubmed|33481774]
Protein family
[wiki|MscS (TC 1.A.23) family] (according to UniProt)
Structure
[PDB|3UDC] (from Caldanaerobacter subterraneus subsp. tengcongensis, 36% identity) [pubmed|23074248]
[AF|O35043]
[wiki|Localization]
cell membrane [Pubmed|19252899]
Expression and Regulation
Operons
genes
[gene|62718BBECEB18244B5CC877509679F5F9A0C50CD|yfkA]-[gene|5ACE71EBBDCEB0C534A91DE303FD8069BEFDD6A1|yfkC]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-C271 (yfkC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2269 NBRP B. subtilis, Japan]
1A959 ( ''yfkC''::''tet''), [Pubmed|18310427], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A959&Search=1A959 BGSC]
BKE07940 ([gene|5ACE71EBBDCEB0C534A91DE303FD8069BEFDD6A1|yfkC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTAAGTGTTTCTTTCATCC, downstream forward: _UP4_TAAACAAAAAAACTGATTCC
BKK07940 ([gene|5ACE71EBBDCEB0C534A91DE303FD8069BEFDD6A1|yfkC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTAAGTGTTTCTTTCATCC, downstream forward: _UP4_TAAACAAAAAAACTGATTCC
GP2785 (Δ[gene|5ACE71EBBDCEB0C534A91DE303FD8069BEFDD6A1|yfkC]::kan) , available in [wiki|Jörg Stülke]'s lab
GP3131 ([gene|5ACE71EBBDCEB0C534A91DE303FD8069BEFDD6A1|yfkC]-R42W kan [gene|FC05D5FC95CEDC4B4A04040C86170D185528B9FF|plsC]-L48P phleo) , available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
Labs working on this gene/protein
[wiki|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]
Page visits: 3302
Time of last update: 2025-04-16 00:53:17
Author of last update: Jstuelk