citZ
168
[metabolite|citrate] synthase
locus
BSU_29140
Molecular weight
41.57 kDa
pI
5.45
function
TCA cycle
product
[metabolite|citrate] synthase II
essential
no
ec
2.3.3.1
synonyms
citZ, citA2
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0372 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,981,151 2,982,269
Phenotypes of a mutant
[metabolite|glutamate] auxotrophy and a defect in sporulation [Pubmed|8045898]
The protein
Catalyzed reaction/ biological activity
[metabolite|acetyl-CoA] + H2O + [metabolite|oxaloacetate] --> [metabolite|citrate] + [metabolite|CoA] + H+ (according to UniProt)
Protein family
citrate synthase family (with [protein|3DB116F7423C31838ED6EE090B94DA5F93A4F40C|mmgD] and [protein|6684F6083B001E9FDC5967799B715AE966B83E1D|citA], according to UniProt)
Structure
[PDB|1IOM] (from Thermus thermophilus, 48& identity) [pubmed|27493854]
[PDB|2C6X] ([protein|6684F6083B001E9FDC5967799B715AE966B83E1D|citA], 42% identity)
[PDB|A discussion of citrate synthase structure]
[AF|P39120]
Modification
phosphorylation on Ser-284 [Pubmed|17218307]
Effectors of protein activity
Inhibited by acetyl-CoA, 2-oxoglutarate and NADH [Pubmed|4211224] [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01/01/1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(#toc#4938#1985#998209998#270526#FLP#display#Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]
Inhibited by citrate and CoA (competitively against acetyl-CoA and non-competitively against oxaloacetate) [Pubmed|4211224]
Inhibited by ATP competitively in ''B. subtilis'' strain 168 and HS 1A17 [Pubmed|4980242] [Pubmed|4211224]
In ''B. subtilis'' strain HS 2A2, ATP inhibits a non-competitive fashion [Pubmed|4211224]
Activated by AMP [Pubmed|4211224]
Paralogous protein(s)
[protein|6684F6083B001E9FDC5967799B715AE966B83E1D|citA], [protein|3DB116F7423C31838ED6EE090B94DA5F93A4F40C|mmgD]
[wiki|Localization]
cytoplasm (homogeneously distributed throughout the cell) [Pubmed|24825009]
Additional information
[protein|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ] is completely degraded in the forespore by [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP] using [protein|7CFDFB42AF816FF5766086E537472008E6CB7E85|mdfA] as the adaptor protein [pubmed|39651166]
extensive information on the structure and enzymatic properties of CitZ can be found at [http://www.proteopedia.org/wiki/index.php/Citrate_synthase Proteopedia]
Expression and Regulation
Operons
genes
[gene|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ]-[gene|CB7D0E32C044D541CD966DC1F9DA489D518A7703|icd]-[gene|5832127A2D0BD4AF2E891C93DC7C45221055FFC5|mdh]
description
[Pubmed|8045899]
regulation
''[protein|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ]'': catabolite repression ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|12100558]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [protein|FE15A41A58A8177280817CA3825764C39185021A|ccpC]: transcription repression [Pubmed|12100558], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|FE15A41A58A8177280817CA3825764C39185021A|ccpC]: repression, (molecular inducer: citrate) [Pubmed|10656796], in [regulon|protein:FE15A41A58A8177280817CA3825764C39185021A|ccpC regulon]
[protein|E15E9F88B0E03FE8834C646369D2F4B4E713EF41|citB]: mRNA stability control, upon citrate accumulation or iron limitation [Pubmed|23354745], in [regulon|protein:E15E9F88B0E03FE8834C646369D2F4B4E713EF41|citB regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8045899], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP678 (erm), GP797 (spec) available in [wiki|Jörg Stülke]'s lab
1A999 (''citZ''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A999&Search=1A999 BGSC]
GP790 Δ(''[gene|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ]-[gene|CB7D0E32C044D541CD966DC1F9DA489D518A7703|icd]-[gene|5832127A2D0BD4AF2E891C93DC7C45221055FFC5|mdh]'')::''kan'', available in [wiki|Jörg Stülke]'s lab
GP797 (''citZ''::''spec''), allows expression of ''[gene|CB7D0E32C044D541CD966DC1F9DA489D518A7703|icd]'' and ''[gene|5832127A2D0BD4AF2E891C93DC7C45221055FFC5|mdh]'', available in [wiki|Jörg Stülke]'s lab
GP1281 (''citZ''::''erm''), allows expression of ''[gene|CB7D0E32C044D541CD966DC1F9DA489D518A7703|icd]'' and ''[gene|5832127A2D0BD4AF2E891C93DC7C45221055FFC5|mdh]'', available in [wiki|Jörg Stülke]'s lab
GP2331 Δ(''[gene|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ]-[gene|CB7D0E32C044D541CD966DC1F9DA489D518A7703|icd]-[gene|5832127A2D0BD4AF2E891C93DC7C45221055FFC5|mdh]'')::''kan'', Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'',available in [wiki|Jörg Stülke]'s lab
GP2333 Δ(''[gene|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ]-[gene|CB7D0E32C044D541CD966DC1F9DA489D518A7703|icd]-[gene|5832127A2D0BD4AF2E891C93DC7C45221055FFC5|mdh]'')::''lox72'', Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [wiki|Jörg Stülke]'s lab
BKE29140 ([gene|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAACATCTCCTTTT, downstream forward: _UP4_TAAAGAACCATTGGAGGCTG
BKK29140 ([gene|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAACATCTCCTTTT, downstream forward: _UP4_TAAAGAACCATTGGAGGCTG
Expression vectors
pGP1120 (N-terminal Strep-tag, for [wiki|SPINE], purification from ''B. subtilis'', in [wiki|pGP380]) (available in [wiki|Jörg Stülke]'s lab) [pubmed|20933603]
pGP1776 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab)
pGP1761 (expression with N-terminal His-tag from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP2515 (N-terminal Strep-tag, purification from ''E. coli'', in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
pGP2261 (integration into [gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA], expression under the control of the xylose-inducible PxylA promoter in ''B. subtilis'', in [wiki|pGP888]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
Antibody
available in [wiki|Linc Sonenshein]'s lab
labs
[wiki|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
[wiki|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
References
Reviews
Original Publications
Page visits: 9107
Time of last update: 2024-12-20 23:37:46
Author of last update: Jstuelk