thrD
168
aspartokinase III
locus
BSU_03790
Molecular weight
49.66 kDa
pI
4.78
function
biosynthesis of threonine
product
aspartokinase III
essential
no
ec
2.7.2.4
synonyms
thrD, yclM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0527 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
430,623 431,987
The protein
Catalyzed reaction/ biological activity
ATP + L-aspartate --> 4-phospho-L-aspartate + ADP (according to UniProt)
Protein family
aspartokinase family (with [protein|6D6718AC72C9D86EDE16B191EDAB3ABA488B689B|dapG] and [protein|F7B947938FB40E54D89A719E9610C5A4AA400674|lysC], according to UniProt)
[wiki|Domains]
2 [wiki|ACT domain]s (aa 312-388, aa 389-454) (according to UniProt)
Structure
[PDB|3TVI] (from Clostridium acetobutylicum, 37% identity) [pubmed|25170437]
[AF|P94417]
Effectors of protein activity
inhibited by the simultaneous presence of threonine and lysine [Pubmed|11471740]
Expression and Regulation
Operons
genes
[gene|548E92526BC00999031D6EDE43FE061CE0448AFC|thrD]
description
[Pubmed|22383849]
regulation
expressed in the presence of lysine or cysteine ([wiki|ThrR]) [Pubmed|27260660]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|23569278], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]: repression, [Pubmed|27260660], in [regulon|protein:A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR regulon]
Biological materials
Mutant
MGNA-C009 (yclM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2007 NBRP B. subtilis, Japan]
BKE03790 (Δ[gene|548E92526BC00999031D6EDE43FE061CE0448AFC|thrD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTACATCTCCTAATG, downstream forward: _UP4_TAATCGTACATAAATAGCGG
BKK03790 (Δ[gene|548E92526BC00999031D6EDE43FE061CE0448AFC|thrD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTACATCTCCTAATG, downstream forward: _UP4_TAATCGTACATAAATAGCGG
lacZ fusion
pBP318 (in [wiki|pAC5]), available in [wiki|Fabian Commichau]'s lab [Pubmed|27260660]
References
Reviews
Original Publications
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Time of last update: 2025-04-07 23:43:42
Author of last update: Jstuelk