mrfA
168
ATP-dependent helicase, repair of mitomycin C-induced DNA lesions, part of a novel nucleotide excision repair pathway
locus
BSU_22220
Molecular weight
84.40 kDa
pI
6.16
function
repair of mitomycin C-induced DNA lesions
product
3′-to-5′ DNA helicase
essential
no
synonyms
mrfA, yprA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1205 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,334,581 2,336,830
Phenotypes of a mutant
sensitive to mitomycin C [pubmed|30379365]
The protein
Catalyzed reaction/ biological activity
3′-to-5′ DNA helicase [pubmed|33300032]
Protein family
[wiki|helicase family] (according to UniProt)
[wiki|Domains]
[wiki|Helicase ATP-binding domain] ([protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-like) (aa 40-250) [pubmed|33300032]
[wiki|Helicase C-terminal domain] ([protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-like) (aa 251-423) [pubmed|33300032]
Winged helix domain (aa 424-490) [pubmed|33300032]
Zn2+-binding domain (aa 631-749) [pubmed|33300032]
[wiki|Cofactors]
Zn2+ [pubmed|33300032]
Structure
[PDB|6ZNS] (apo-MrfA) [pubmed|33300032]
[PDB|6ZNP] (MrfA-DNA) [pubmed|33300032]
[AF|P50830]
Expression and Regulation
Operons
genes
[gene|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA]-[gene|3F1D78CAAA4B5057DEBB24C4D382F96DA4AA4852|mrfB]-[gene|FA8D9AA0968365522DEFC05B277123FC96482044|cotD]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A412 (yprA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/412 NBRP B. subtilis, Japan]
BKE22220 ([gene|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTTACACCTCTTTGT, downstream forward: _UP4_ATGTCGTAAGGAGGGAGGCC
BKK22220 ([gene|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTTACACCTCTTTGT, downstream forward: _UP4_ATGTCGTAAGGAGGGAGGCC
References
Reviews
Research papers
Page visits: 2076
Time of last update: 2025-01-31 01:27:37
Author of last update: Jstuelk