efp
168
elongation factor P, important for the [category|SW.3.3.1|Translation] of proteins containing three or more consecutive proline residues
locus
BSU_24450
Molecular weight
20.31 kDa
pI
4.84
function
[category|SW.3.3.1|Translation]
product
elongation factor P
essential
no
synonyms
efp, yqhU, yqgF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0231 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,538,115 2,538,672
Phenotypes of a mutant
defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827,15066026,27002156]
inactivation of ''[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]'' reduces sporulation efficiency to 1% that of wild type cells [Pubmed|26735940]
an [gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] [gene|F69F15198ACF8A8347C450EEC09F000EECEDEC41|yfmR] double mutant is not viable [pubmed|37577462]
The protein
Catalyzed reaction/ biological activity
see a list of [category|SW.6.11|Efp-dependent proteins]
Protein family
elongation factor P family (single member, according to UniProt)
Structure
[PDB|6RK3] (from Staphylococcus aureus, 71% identity) [pubmed|32152681]
[AF|P49778]
Modification
carries a 5-aminopentanol moiety at Lys-32, this modification is essential for activity [Pubmed|27002156]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
Expression and Regulation
Operons
genes
[gene|449EEBF33F52B461F244331ACD140E68CF64EE7C|figP]-[gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]-[gene|33886A1EAC13B684E92BF94201C46617AD2844B3|papA]-[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]
description
[pubmed|22383849]
genes
[gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]-[gene|33886A1EAC13B684E92BF94201C46617AD2844B3|papA]-[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-C464 (efp::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2462 NBRP B. subtilis, Japan]
BKE24450 (''[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]''::''erm'', available in the BGSC and in [wiki|Jörg Stülke]'s lab) [pubmed|28189581]
BKE24450 ([gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTAATGTCCTCCTAT, downstream forward: _UP4_TAGAAAGAAAAAAAGAAGTC
BKK24450 ([gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTAATGTCCTCCTAT, downstream forward: _UP4_TAGAAAGAAAAAAAGAAGTC
References
Reviews
Original Publications
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Time of last update: 2024-12-09 22:05:22
Author of last update: Jstuelk