ntdC
168
NAD-dependent glucose-6-phosphate dehydrogenase
locus
BSU_10530
Molecular weight
39.14 kDa
pI
6.23
function
synthesis of the antibiotic kanosamine
product
NAD-dependent glucose-6-phosphate dehydrogenase
essential
no
synonyms
ntdC, yhjJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0673 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,126,400 1,127,452
The protein
Catalyzed reaction/ biological activity
glucose-6-phosphate + NAD - 3-oxo-d-glucose-6-phosphate + NADH(2) [Pubmed|23586652]
Protein family
[wiki|Gfo/Idh/MocA family] (according to UniProt)
[wiki|Cofactors]
NAD+ [Pubmed|23586652]
Structure
[PDB|3EUW] (from Corynebacterium glutamicum, 21% identity)
[AF|O07564]
Paralogous protein(s)
[protein|484D17480DFAD3667E0EBC6D38854A7546A8DB46|yteT], [protein|7DFE74C751A67CCC84579D52005E1399B0A10166|iolW], [protein|920C5750CB95102B073C32874D512E8D636D2C7D|iolU], [protein|93C1DF93CAFC5AE726523A91A3BA7470017FF6DC|iolG], [protein|942BAE9FA023DFCA06B6D26A856A72F2979E23FA|yrbE], [protein|AA7DCEFB17FD6F9C4F65235E9A247A6EC2242B06|iolX], [protein|03BE6DA854612B3F6741E0E167AB310F0DE96285|yfiI]
Expression and Regulation
Operons
genes
[gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]-[gene|A4DE6C394B64813A732255734758403241FFAC2F|ntdB]-[gene|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|ntdC]-[gene|ADB476B0B385D9B9CAF02902CE115C8309F67911|glcP]
description
[Pubmed|17056753]
regulation
induced by 3,3'-neotrehalosadiamine and kanosamine ([protein|9118A36CD30E7C99697B3659BB04E03BB7EE71D7|ntdR]) [Pubmed|33619155,14612444]
regulatory mechanism
[protein|9118A36CD30E7C99697B3659BB04E03BB7EE71D7|ntdR]: activation , in [regulon|protein:9118A36CD30E7C99697B3659BB04E03BB7EE71D7|ntdR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|14612444], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B286 (yhjJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1285 NBRP B. subtilis, Japan]
BKE10530 ([gene|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|ntdC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTATTTCCTCCTCAT, downstream forward: _UP4_TAGTATTTCAAAGAGAGAAG
BKK10530 ([gene|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|ntdC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTATTTCCTCCTCAT, downstream forward: _UP4_TAGTATTTCAAAGAGAGAAG
References
Reviews
Original Publications
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Time of last update: 2025-01-17 20:20:02
Author of last update: Jstuelk