metE
168
methionine synthase
locus
BSU_13180
Molecular weight
86.64 kDa
pI
4.84
function
biosynthesis of methionine
product
methionine synthase
essential
no
ec
2.1.1.14
synonyms
metE, metC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0620 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,383,320 1,385,608
The protein
Catalyzed reaction/ biological activity
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine --> L-methionine + tetrahydropteroyltri-L-glutamate (according to UniProt)
Protein family
vitamin-B12 independent methionine synthase family (single member, according to UniProt)
Structure
[PDB|1T7L] (from ''Thermotoga maritima'', 44% identity, 61% similarity) [Pubmed|15630480]
[AF|P80877]
Modification
phosphorylated on ser/ thr/ tyr [Pubmed|17726680], S-cysteinylation after diamide stress (C719) [Pubmed|17611193]
Cys719 and Cys730 are S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species [Pubmed|21749987] [Pubmed|22938038]
MetE is generally most strongly S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species [Pubmed|21749987] [Pubmed|22938038]
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|23F405D5B849E4EDC1D599F86C71DFD2E55C0305|metE]
regulation
repressed by casamino acids [Pubmed|12107147]
the [wiki|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
S-box: RNA switch, , the [wiki|S-box] [wiki|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|other_regulator:S-box|S-box]
Biological materials
Mutant
1A607 ( ''metE''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A607&Search=1A607 BGSC]
BKE13180 ([gene|23F405D5B849E4EDC1D599F86C71DFD2E55C0305|metE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTTCTCCTCCTTTAT, downstream forward: _UP4_TAATTTGAAAAAACCATCTG
BKK13180 ([gene|23F405D5B849E4EDC1D599F86C71DFD2E55C0305|metE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTTCTCCTCCTTTAT, downstream forward: _UP4_TAATTTGAAAAAACCATCTG
References
Reviews
Original Publications
Page visits: 3983
Time of last update: 2025-04-04 06:30:41
Author of last update: Melvin.boenninger