rae1
168
[wiki|ribosome]-associated A site endoribonuclease
locus
BSU_00970
Molecular weight
19.53 kDa
pI
7.28
function
ribosome-dependent mRNA decay
product
A site endoribonuclease
essential
no
synonyms
yacP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3688 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
116,025 116,537
The protein
[wiki|Domains]
N-terminal NYN domain [pubmed|28363943,17114934]
C-terminal flexible RNA-binding domain [pubmed|28363943]
Structure
[PDB|5MQ8] [pubmed|28363943]
[AF|P37574]
Expression and Regulation
Operons
genes
[gene|132E91DB8C81CD8C4F5160B2C068B74DA10AE224|gltX]-[gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE]-[gene|E5826A99769E2FE45959A9D623D6467A8DDDEB81|cysS]-[gene|031FDF8443A8A4D5B042B7591EBE2E6C74F07702|mrnC]-[gene|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]-[gene|172652156ABFE72D5BAAC22729CE17CA947538B5|rae1]
description
[Pubmed|7510287]
regulation
expression transiently increases in the forespore [Pubmed|22848659]
the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
T-box: RNA switch, overlaps a transcription terminator upstream of [gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE], in [regulon|other_regulator:T-box|T-box]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7510287], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B885 (yacP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1884 NBRP B. subtilis, Japan]
BKE00970 ([gene|172652156ABFE72D5BAAC22729CE17CA947538B5|rae1]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGGTCTTTATTCTCCCA, downstream forward: _UP4_TAAGTTGACGCTTTTTTGCC
BKK00970 ([gene|172652156ABFE72D5BAAC22729CE17CA947538B5|rae1]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGGTCTTTATTCTCCCA, downstream forward: _UP4_TAAGTTGACGCTTTTTTGCC
References
Reviews
Original Publications
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Time of last update: 2025-04-03 10:53:09
Author of last update: Jstuelk