sigL
168
[wiki|sigma factor ]of the [wiki|RNA polymerase], Sigma-54, Sigma L
locus
BSU_34200
Molecular weight
49.54 kDa
pI
7.79
function
utilization of arginin, acetoin and fructose, required for cold adaptation
product
[wiki|RNA polymerase] sigma-54 factor (sigma-L)
essential
no
synonyms
sigL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1508 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,512,498 3,513,808
Phenotypes of a mutant
The mutant is cold-sensitive and unable to use arginine as a single carbon source [pubmed|16585774]
The protein
Catalyzed reaction/ biological activity
Binding to promoters of the -12, -24 type
Protein family
sigma-54 factor family (single member, according to UniProt)
[wiki|Domains]
DNA binding domain (HTH motif) (324343)
pron box domain (413421)
3 x Compositional bias domain (621),(3253),(112136)
Structure
[PDB|5BYH] (''E. coli'' [wiki|RNA polymerase] containing Sigma-54) [Pubmed|26293966]
[AF|P24219]
Additional information
Transcription initiation by SigL-containing [wiki|RNA polymerase] requires the activity of ATP-hydrolyzing transcription activators
information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000078 PRODORIC2 database]
Expression and Regulation
Operons
genes
[gene|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]
description
[Pubmed|22383849]
regulation
repressed by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|16166551]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, transcriptional roadblock [Pubmed|16166551], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
Biological materials
Mutant
GP146 (Δ''sigL''::spc), available in [wiki|Jörg Stülke]'s lab [Pubmed|17183217]
GP2302 (Δ''sigL''::tet), available in [wiki|Jörg Stülke]'s lab
1A914 (''sigL''::''kan''), [Pubmed|16585774], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A914&Search=1A914 BGSC]
BKE34200 (Δ[gene|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTGCTCACTCCCCTTT, downstream forward: _UP4_TAAAATCCTCCCTAGACGGG
BKK34200 (Δ[gene|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTGCTCACTCCCCTTT, downstream forward: _UP4_TAAAATCCTCCCTAGACGGG
Expression vectors
pGP3707: expression of Strep-''sigL'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP3714: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
pGP3798 for the construction of ''sigL-3xFLAG spc'' (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-24 09:05:50
Author of last update: Jstuelk