azoR2

azoR2
168

similar to NAD(P)H dehydrogenase (quinone)

Locus
BSU_33540
Molecular weight
23.13 kDa
Isoelectric point
5.11
Protein length
211 aaSequenceBlast
Gene length
636 bpSequenceBlast
Function
resistance to 2-methylhydroquinone
Product
azoreductase
Essential
no
Synonyms
azoR2, yvaB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1182 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,445,442  3,446,077
The protein
Catalyzed reaction/ biological activity
anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ --> 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 H+ + 2 NADH (according to UniProt)
Protein family
azoreductase type 1 family (with AzoR1, according to UniProt)
Structure
3P0R (PDB) (from B. anthracis, 43% identity, 61% similarity)
O32224 (AlphaFold)
Paralogous protein(s)
AzoR1
Expression and Regulation
Operons
Genes
azoR2
Description
PubMed
Regulation
induced by catechol and 2-methylhydroquinone (2-MHQ) (MhqR) PubMed
Regulatory mechanism
MhqR: repression, PubMed, in mhqR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigG: sigma factor, PubMed, in sigG regulon
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azoR2

2025-03-11 11:22:15

ghost

64

1a7a57d5b4b939a6aa2c8086b52e823c3b3bc93b

3EE588FB5273FB7C3410D218ACC658D1AFC37565

Biological materials
Mutant
MGNA-A444 (yvaB::erm), available at the NBRP B. subtilis, Japan
BKE33540 (azoR2::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTACCAGCCCTTTCG,  downstream forward: _UP4_TAATGAAAAAGCCTCCCCTT
BKK33540 (azoR2::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTACCAGCCCTTTCG,  downstream forward: _UP4_TAATGAAAAAGCCTCCCCTT
References
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0E2B1914A040666BAF042C0BE3F468144E1F1E06

Page visits: 4108

Time of last update: 2025-03-16 18:00:18

Author of last update: Melvin.boenninger