azoR2
168
similar to NAD(P)H dehydrogenase (quinone)
locus
BSU_33540
Molecular weight
23.13 kDa
pI
5.11
function
resistance to 2-methylhydroquinone
product
azoreductase
essential
no
synonyms
azoR2, yvaB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1182 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,445,442 3,446,077
The protein
Catalyzed reaction/ biological activity
anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ --> 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 H+ + 2 NADH (according to UniProt)
Protein family
azoreductase type 1 family (with [protein|CFFFFE96FB7AEEF7190F81332369F0A6834C34A4|azoR1], according to UniProt)
Structure
[PDB|3P0R] (from B. anthracis, 43% identity, 61% similarity)
[AF|O32224]
Paralogous protein(s)
[protein|CFFFFE96FB7AEEF7190F81332369F0A6834C34A4|azoR1]
Expression and Regulation
Operons
genes
[gene|0E2B1914A040666BAF042C0BE3F468144E1F1E06|azoR2]
description
[Pubmed|17725564]
regulation
induced by catechol and 2-methylhydroquinone (2-MHQ) ([protein|search|MhqR]) [Pubmed|17725564]
regulatory mechanism
[protein|997B828A99D6D8460712C18ED0CE566B285C22DC|mhqR]: repression, [Pubmed|17725564], in [regulon|protein:997B828A99D6D8460712C18ED0CE566B285C22DC|mhqR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|17725564], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|16497325], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
Biological materials
Mutant
MGNA-A444 (yvaB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/444 NBRP B. subtilis, Japan]
BKE33540 ([gene|0E2B1914A040666BAF042C0BE3F468144E1F1E06|azoR2]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTACCAGCCCTTTCG, downstream forward: _UP4_TAATGAAAAAGCCTCCCCTT
BKK33540 ([gene|0E2B1914A040666BAF042C0BE3F468144E1F1E06|azoR2]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTACCAGCCCTTTCG, downstream forward: _UP4_TAATGAAAAAGCCTCCCCTT
References
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Time of last update: 2025-03-17 13:12:45
Author of last update: Melvin.boenninger