pstBB
168
phosphate [wiki|ABC transporter ]([metabolite|ATP]-binding protein)
locus
BSU_24950
Molecular weight
29.05 kDa
pI
7.77
function
high-affinity phosphate uptake
product
phosphate [wiki|ABC transporter] (ATP-binding protein)
essential
no
ec
3.6.3.27
synonyms
pstBB, pstB, yqgK, yzmF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1117 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,577,210 2,577,992
The protein
Catalyzed reaction/ biological activity
ATP + H2O + phosphate --> ADP + H+ + 2 phosphate (according to UniProt)
Protein family
[wiki|ABC transporter superfamily] (according to UniProt)
[wiki|Domains]
[wiki|ABC transporter domain] (aa 8-255) (according to UniProt)
Structure
[PDB|4YMS] (from Caldanaerobacter subterraneus, 38% identity) [pubmed|25848002]
[AF|P46342]
Paralogous protein(s)
[protein|434BCED6953BE07D592AC229264C5AD0D04CBAF6|pstBA], [protein|468BEA9EAEA589D683DB79B2C689A5C6A22AB67F|glnQ], [protein|6882EF93EC82872B25C88104F62D6603D2514890|artR], [protein|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN], [protein|B453986691E1231A1C565D469A2A60EBFF2F6BD3|tcyC], [protein|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO]
Expression and Regulation
Operons
genes
[gene|BA45631351E3C9C140D15F620D7B7AC143C04932|pstS]-[gene|8BC7CCE9EBD899912ED7C72CA04FE82B21516F77|pstC]-[gene|5CE86C6768A4EF313FB5DBC270AE2A773EA58E81|pstA]-[gene|434BCED6953BE07D592AC229264C5AD0D04CBAF6|pstBA]-[gene|0D5BC94E21D51373A50D61CE3D0895B3DB28F6B3|pstBB]
description
[Pubmed|9098050]
regulation
expressed under conditions of phosphate limitation (5000-fold induced) ([protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9098050,9593301 PubMed]
regulatory mechanism
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]-P, [PubMed|9098050,9593301], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9098050], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
mRNA processing between [gene|BA45631351E3C9C140D15F620D7B7AC143C04932|pstS] and [gene|8BC7CCE9EBD899912ED7C72CA04FE82B21516F77|pstC] to maintain higher concentrations of [protein|BA45631351E3C9C140D15F620D7B7AC143C04932|pstS] relative to other components of the [wiki|ABC transporter] [PubMed|15289558]
Biological materials
Mutant
MGNA-C450 (yqgK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2448 NBRP B. subtilis, Japan]
BKE24950 ([gene|0D5BC94E21D51373A50D61CE3D0895B3DB28F6B3|pstBB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTAGCAATACTCATTGCCG, downstream forward: _UP4_TGAAAAAAACAGGCTGAGTC
BKK24950 ([gene|0D5BC94E21D51373A50D61CE3D0895B3DB28F6B3|pstBB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTAGCAATACTCATTGCCG, downstream forward: _UP4_TGAAAAAAACAGGCTGAGTC
labs
[wiki|Marion Hulett], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]
References
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Time of last update: 2024-12-19 02:56:10
Author of last update: Jstuelk