swrAA/2
168
control of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU] activity, enhances [gene|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD] transcription, part of the [gene|5D479874B43F521DB52EDC2C27CDE4967F22DE47|swrA] pseudogene
locus
BSU_35239
function
essential for swarming differentiation on solid surfaces
product
swarming motility protein
essential
no
synonyms
swrAA/2
Outlinks
Genomic Context
Categories containing this gene/protein
This gene is a member of the following regulons
Gene
Coordinates
3,621,931 3,622,047
additional information
in the laboratory strain 168, the [gene|5D479874B43F521DB52EDC2C27CDE4967F22DE47|swrA] gene contains a frameshift mutation (insertion of an extra A in a stretch of eight As), thus the gene is annotated as [gene|0CEE58D799AF41634D161DBDF3D67EEFFF1C861E|swrAA/2] (corresponds to the small N-terminal part of the protein) and [gene|5D479874B43F521DB52EDC2C27CDE4967F22DE47|swrA] (corresponds to the large C-terminal part of the protein) [pubmed|15066026]
the mutation readily reverts giving rise to swarming behaviour [pubmed|15066026]
the [gene|5D479874B43F521DB52EDC2C27CDE4967F22DE47|swrA] gene is intact in wild type strains such as NCIB3610 [pubmed|15066026]
Expression and Regulation
Operons
genes
[gene|0CEE58D799AF41634D161DBDF3D67EEFFF1C861E|swrAA/2]-[gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ]
description
[Pubmed|16030230]
regulatory mechanism
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P, ([protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA] promoter) [Pubmed|18567663], in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|18567663], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|18567663], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
genes
[gene|5D479874B43F521DB52EDC2C27CDE4967F22DE47|swrA]-[gene|0CEE58D799AF41634D161DBDF3D67EEFFF1C861E|swrAA/2]
description
[pubmed|22383849]
regulatory mechanism
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P, ([protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA] promoter) [Pubmed|18567663], in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|18567663], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|18567663], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
SwrA levels are 3 ... 10-fold increased on solid medium as compared to liquid medium (due to [protein|FF15BE26BCC78EC1301C58A51AF0A519D7BE9ADC|lonA]/[protein|A6E7212D159F076F5D26F9C02F340B40C3667623|smiA]-mediated degradation of SwrA in liquid medium) [PubMed|25538299]
Biological materials
Mutant
BKE35239 ([gene|0CEE58D799AF41634D161DBDF3D67EEFFF1C861E|swrAA/2]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35239 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCGCTAACGTTACTC, downstream forward: _UP4_GGTTCACAATTGAAGAGGGC
BKK35239 ([gene|0CEE58D799AF41634D161DBDF3D67EEFFF1C861E|swrAA/2]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35239 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCGCTAACGTTACTC, downstream forward: _UP4_GGTTCACAATTGAAGAGGGC
labs
see [protein|5D479874B43F521DB52EDC2C27CDE4967F22DE47|swrA]
References
see [protein|5D479874B43F521DB52EDC2C27CDE4967F22DE47|swrA]
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Time of last update: 2024-12-23 13:27:59
Author of last update: Jstuelk