metI
168
O-succinylhomoserine lyase (L-cysteine, H2S, methanethiol, elimination)
locus
BSU_11870
Molecular weight
41.55 kDa
pI
5.02
function
biosynthesis of methionine
product
O-succinylhomoserine lyase (L-cysteine, H2S, methanethiol, elimination)
essential
no
synonyms
metI, yjcI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0626 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,258,492 → 1,259,613
The protein
Catalyzed reaction/ biological activity
L-cysteine + O-acetyl-L-homoserine --> acetate + H+ + L,L-cystathionine (according to UniProt)
hydrogen sulfide + O-acetyl-L-homoserine --> acetate + L-homocysteine (according to UniProt)
Protein family
trans-sulfuration enzymes family (with [protein|314CB4923D15140E72B0ABAFE3E8BE9CA7310E10|mccB] and [protein|1C0D38F23D3A2CAA1EB057A92D6F571D0D2AA724|metC], according to UniProt)
[wiki|Cofactors]
PLP (according to UniProt)
Structure
[PDB|4L0O] (from Helicobacter pylori, 48% identity)
[AF|O31631]
Paralogous protein(s)
[protein|1C0D38F23D3A2CAA1EB057A92D6F571D0D2AA724|metC], [protein|314CB4923D15140E72B0ABAFE3E8BE9CA7310E10|mccB]
Expression and Regulation
Operons
genes
[gene|04DC792180FDC5E7916F2DBB2EC2C34369202FE0|metI]-[gene|1C0D38F23D3A2CAA1EB057A92D6F571D0D2AA724|metC]
description
[Pubmed|11832514]
regulation
induced by methionine starvation ([wiki|S-box]) [Pubmed|10094622]
the [wiki|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
S-box: RNA switch, the [wiki|S-box] [wiki|riboswitch] binds S-adenosylmethionine resulting in termination, in [regulon|other_regulator:S-box|S-box]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11832514], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B298 (yjcI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1297 NBRP B. subtilis, Japan]
1A941 ( ''metI''::''spec''), [Pubmed|11832514], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A941&Search=1A941 BGSC]
BKE11870 (Δ[gene|04DC792180FDC5E7916F2DBB2EC2C34369202FE0|metI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAGCTGACACCTTCCC, downstream forward: _UP4_CAGGTCAAAGAGGGAGCTGT
BKK11870 (Δ[gene|04DC792180FDC5E7916F2DBB2EC2C34369202FE0|metI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAGCTGACACCTTCCC, downstream forward: _UP4_CAGGTCAAAGAGGGAGCTGT
References
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Time of last update: 2025-03-16 00:06:13
Author of last update: Jstuelk