degS
168
two-component sensor kinase for exoenzyme and competence regulation, kinase activity is activated in response to inhibition of flagellar rotation
locus
BSU_35500
Molecular weight
44.80 kDa
pI
5.99
function
regulation of degradative enzyme and genetic competence
product
two-component sensor kinase
essential
no
synonyms
degS, sacU
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4585 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,645,379 3,646,536
Phenotypes of a mutant
the mutation suppresses the mucoid phenotype of ''[gene|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA]'' or ''[gene|FE56753D061344B0F10A6523F49C5C7356AA40B6|motB]'' mutants due to loss of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU] phosphorylation and concomitant reduced expression of the ''[gene|86C05126ADBA22BB1771B1FA3D214B2E7A36311E|capB]-[gene|D66994D077D1382B88FF65075E44C2A31089548F|capC]-[gene|A696434A086A428D411CAF45B37CD8F82AC2503F|capA]-[gene|6B61DCE8D27776EBF942621D3AE90F410FD13F37|capE]'' operon [Pubmed|24296669]
The protein
Catalyzed reaction/ biological activity
autophosphorylation, autodephosphorylation, phosphorylation of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU] on Asp-56 [Pubmed|1901568]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
[wiki|Domains]
aa 12 ... 170: unknown DegS domain
[wiki|Histidine kinase domain] (aa 183-385) (according to UniProt)
aa 289 ... 384: HATPase_c
Structure
[PDB|3GIG] ([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK], corresponds to the HisKA_3 and HATPase_c domains, 28% identity) [pubmed|19805278]
[AF|P13799]
Modification
phosphorylation on Ser-76 [Pubmed|17218307], this stimulates phosphotransfer from His-189 to [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU] [Pubmed|21304896], the phosphorylyation is performed by [protein|E0097A00C82136BB0B1FB89FBA177E28A4EC3D54|prkD] ''in vitro'' [Pubmed|21304896]
autophosphorylation on His-189
Effectors of protein activity
[protein|40C1E81BAB04BD1CC98FC57DF25D27DBDFEB5A59|degQ] (stimulation of autophosphorylation) [Pubmed|21965392]
inhibition of flagellar rotation stimulates kinase activity [Pubmed|23888912]
[wiki|Localization]
Cytoplasm (Homogeneous) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|047CE33C0CC127DAD15D68D63843DF89F93ED3BF|degS]-[gene|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]
description
[Pubmed|1688843]
regulation
induced by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|23123903]
regulatory mechanism
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: repression, [Pubmed|18502860], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: activation, [Pubmed|23123903], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]: repression, [Pubmed|24317403], in [regulon|protein:5A6FBAE6553343092862CB79E150F934978C32A9|sinR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1688843], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE35500 ([gene|047CE33C0CC127DAD15D68D63843DF89F93ED3BF|degS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTTCCCTCCGTCACG, downstream forward: _UP4_TGACTATGATTTGTAAAATA
BKK35500 ([gene|047CE33C0CC127DAD15D68D63843DF89F93ED3BF|degS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTTCCCTCCGTCACG, downstream forward: _UP4_TGACTATGATTTGTAAAATA
lacZ fusion
GP1982 ([gene|047CE33C0CC127DAD15D68D63843DF89F93ED3BF|degS]::lacZ cat, based on [wiki|pAC5]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Tarek Msadek], Institut Pasteur, Paris, France
References
Reviews
Original Publications
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Time of last update: 2024-12-23 23:04:34
Author of last update: Jstuelk