mrnC
168
RNase Mini-III
locus
BSU_00950
Molecular weight
16.09 kDa
pI
9.15
function
maturation of 23S rRNA
product
RNase Mini-III
essential
no
synonyms
mrnC, yazC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1939 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
114,854 115,285
The protein
Protein family
MrnC RNase family (single member, according to UniProt)
Structure
[PDB|4OUN] [pubmed|25634891]
[AF|O31418]
Effectors of protein activity
[protein|FEF94BD8482A41C493A8BEC3278B518E8BB4EDFE|rplC] binding to the precursor 23S rRNA stimulates MrnC activity [Pubmed|19154332]
Expression and Regulation
Operons
genes
[gene|132E91DB8C81CD8C4F5160B2C068B74DA10AE224|gltX]-[gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE]-[gene|E5826A99769E2FE45959A9D623D6467A8DDDEB81|cysS]-[gene|031FDF8443A8A4D5B042B7591EBE2E6C74F07702|mrnC]-[gene|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]-[gene|172652156ABFE72D5BAAC22729CE17CA947538B5|rae1]
description
[Pubmed|7510287]
regulation
expression transiently increases in the forespore [Pubmed|22848659]
the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
T-box: RNA switch, overlaps a transcription terminator upstream of [gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE], in [regulon|other_regulator:T-box|T-box]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7510287], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B883 (yazC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1882 NBRP B. subtilis, Japan]
BKE00950 ([gene|031FDF8443A8A4D5B042B7591EBE2E6C74F07702|mrnC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCGTATCAAATTCAAGCA, downstream forward: _UP4_TTCGGGACGTCAGGGAGGAA
BKK00950 ([gene|031FDF8443A8A4D5B042B7591EBE2E6C74F07702|mrnC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCGTATCAAATTCAAGCA, downstream forward: _UP4_TTCGGGACGTCAGGGAGGAA
labs
[wiki|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
[wiki|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]
References
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Time of last update: 2025-04-01 00:56:04
Author of last update: Jstuelk