yxeN
168
putative S-(2-succino)cysteine [wiki|ABC transporter] (permease)
locus
BSU_39490
Molecular weight
24.86 kDa
pI
9.48
function
uptake and utilization of S-(2-succino)cysteine
product
putative S-(2-succino)cysteine [wiki|ABC transporter] (permease)
essential
no
synonyms
yxeN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0765 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,058,791 → 4,059,465
The protein
Protein family
[wiki|Binding-protein-dependent transport system permease family] (according to UniProt)
[wiki|Domains]
[wiki|ABC transmembrane type-1 domain] (aa 20-211) (according to UniProt)
Structure
[PDB|4YMS] (amino acid transporter from Caldanaerobacter subterraneus, 39% identity) [pubmed|25848002]
[AF|P54953]
Paralogous protein(s)
[protein|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL], [protein|9400DF25487461E3738DDDC72BDDB1589281409D|tcyB], [protein|1872614A6F7DBF7231348AC3DAE4ED3295ACEB41|yckA]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]-[gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]-[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]-[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]-[gene|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO]-[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]-[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]
description
[Pubmed|10746760]
regulation
repressed in the presence of cysteine ([protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]) [Pubmed|16513748]
regulatory mechanism
[protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16513748], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16513748], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the amount of the mRNA is substantially decreased upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
Biological materials
Mutant
GP4477 (trpC2 Δ[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]::neo), available in [wiki|Jörg Stülke]'s lab
GP4677 (trpC2 Δ[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]::lox72), available in [wiki|Jörg Stülke]'s lab
MGNA-B710 (yxeN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1709 NBRP B. subtilis, Japan]
1A950 ( ''yxeN''::''cat''), [Pubmed|15262924], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A950&Search=1A950 BGSC]
BKE39490 (Δ[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGGTGAGTCACCGCC, downstream forward: _UP4_TACCGGACTTAGAGGTGAAA
BKK39490 (Δ[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGGTGAGTCACCGCC, downstream forward: _UP4_TACCGGACTTAGAGGTGAAA
References
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Time of last update: 2024-12-21 20:31:59
Author of last update: Jstuelk