yuaF
168
NfeD2, role in maintaining membrane integrity during conditions of cellular stress, confers (together with [protein|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]) resistance to cefuroxime
locus
BSU_31020
Molecular weight
18.46 kDa
pI
4.04
function
involved in the control of membrane fluidity
product
NfeD family protein NfeD2
essential
no
synonyms
yuaF, NfeD2
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
3,182,015 → 3,182,539
The protein
Catalyzed reaction/ biological activity
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]-dependent expression of ''[gene|5D5D9A544295DD9B55C75A8CF47AB19935E740C6|fabF]'' and the ''[gene|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF]-[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]-[gene|56345EA776E1C5664EF1F2D1AF5CE3E5F40AD442|yuaI]'' operon result in reduced membrane fluidity [Pubmed|21542858,22178969]
Protein family
NfeD family (with [protein|F6B0D27B434D81B7A680555E4457F266B6206B7D|yqeZ])
Structure
[PDB|2K14] [pubmed|18696230]
[AF|O32077]
[wiki|Localization]
cell membrane, in focal structures [Pubmed|22753055]
Expression and Regulation
Operons
genes
[gene|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF]-[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]-[gene|56345EA776E1C5664EF1F2D1AF5CE3E5F40AD442|yuaI]
description
[Pubmed|9987136]
regulation
expressed upon cell wall stress ([protein|search|SigW]) [Pubmed|9987136]
sigma factors
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|9987136], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
Biological materials
Mutant
MGNA-A212 (yuaF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/212 NBRP B. subtilis, Japan]
BKE31020 (Δ[gene|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTCCTCATCCTTTCTT, downstream forward: _UP4_TAACATAAAAGGAGGAATTT
BKK31020 (Δ[gene|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTCCTCATCCTTTCTT, downstream forward: _UP4_TAACATAAAAGGAGGAATTT
References
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Time of last update: 2024-12-19 04:53:43
Author of last update: Bzhu