luxS
168
S-ribosylhomocysteine lyase, autoinducer-2 production protein, required for surface spreading motility (sliding) of B. subtilis natto and biofilm formation
locus
BSU_30670
Molecular weight
17.57 kDa
pI
5.17
function
methionine salvage
product
S-ribosylhomocysteine lyase
essential
no
ec
4.4.1.21
synonyms
luxS, ytjB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1854 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,137,495 → 3,137,968
The protein
Catalyzed reaction/ biological activity
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine --> (S)-4,5-dihydroxypentane-2,3-dione + L-homocysteine (according to UniProt)
Protein family
luxS family (single member, according to UniProt)
Structure
[PDB|1JQW] (complex with homocysteine), [PDB|1J98] [Pubmed|11601850]
[AF|O34667]
Effectors of protein activity
subject to feedback inhibition [Pubmed|19258532]
Expression and Regulation
Operons
genes
[gene|FF61DC6CA162EEC191CDFC802E721FCBD32EC098|luxS]
description
[Pubmed|9387221]
additional information
subject to feedback inhibition [http://www.ncbi.nlm.nih.gov/sites/entrez/19258532 PubMed]
genes
[gene|F942C0F4618FACEF35ACA629355BAC288556E7B6|ytiB]-[gene|FF61DC6CA162EEC191CDFC802E721FCBD32EC098|luxS]
description
[pubmed|22383849]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|26577401], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
MGNA-B540 (ytjB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1539 NBRP B. subtilis, Japan]
1A953 ( ''luxS''::''cat''), [Pubmed|17056751], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A953&Search=1A953 BGSC]
BKE30670 (Δ[gene|FF61DC6CA162EEC191CDFC802E721FCBD32EC098|luxS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGGCACTCTCCCCTT, downstream forward: _UP4_TAAAATAGAAAGGACCTTTT
BKK30670 (Δ[gene|FF61DC6CA162EEC191CDFC802E721FCBD32EC098|luxS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGGCACTCTCCCCTT, downstream forward: _UP4_TAAAATAGAAAGGACCTTTT
References
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Time of last update: 2024-10-14 08:35:20
Author of last update: Melvin.boenninger