mntR
168
transcriptional regulator, controls manganese import and efflux
locus
BSU_24520
Molecular weight
16.60 kDa
pI
5.63
function
regulation of manganese transport
product
transcriptional regulator (DtxR family)
essential
no
synonyms
mntR, yqhN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1321 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,543,440 → 2,543,868
Phenotypes of a mutant
inactivation of [gene|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR] reduces sporulation efficiency to 8.9% that of wild type cells [Pubmed|26735940]
highly sensitive to Mn(II) intoxication [Pubmed|27748968], this can be suppressed by mutations that inactivate [protein|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH] [pubmed|31964700]
sensitive to iron intoxication due to loss of expression of [gene|28C7CFE5701F6920652BE1973DC7A15D7C088E35|mneP] and [gene|72BF9B934CB5800E156263FF377DED0AD2E920A9|mneS] [pubmed|36748638]
more sensitive to H2O2 [pubmed|36748638]
The protein
Catalyzed reaction/ biological activity
dual regulatory protein, responds to heightened [metabolite|Mn(2+)] availability by repressing the expression of uptake transporters and activating the expression of efflux proteins [pubmed|38979284]
Protein family
DtxR/MntR family (single member, according to UniProt)
[wiki|Domains]
HTH dtxR-type domain (aa 1-63) (according to UniProt)
[wiki|Cofactors]
Mn2+ acts as co-repressor/ co-activator (according to [Pubmed|20408793])
Structure
[PDB|9C4D] complex with [protein|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR] bound to [gene|28C7CFE5701F6920652BE1973DC7A15D7C088E35|mneP] upstream DNA fragment [pubmed|38979284]
[PDB|2F5D] (complex with manganese) [pubmed|16533030]
[PDB|2HYG] (apo-form)
[AF|P54512]
Effectors of protein activity
Mn2+ ions facilitate DNA binding and thus repression or activation of the genes of the [wiki|MntR regulon] [pubmed|36674477]
Expression and Regulation
Operons
genes
[gene|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]
description
[pubmed|22383849]
genes
[gene|19CEB80CF637F1FED7C493EF743B4BFA632C8D44|lipM]-[gene|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-C435 (yqhN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2433 NBRP B. subtilis, Japan]
BKE24520 (Δ[gene|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGAAACCCTCCCAAAAA, downstream forward: _UP4_TAAAAAGGCGGTCTCGAAGG
BKK24520 (Δ[gene|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGAAACCCTCCCAAAAA, downstream forward: _UP4_TAAAAAGGCGGTCTCGAAGG
labs
[wiki|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
[wiki|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-20 15:13:53
Author of last update: Jstuelk