ksgA
168
rRNA adenine dimethyltransferase
locus
BSU_00420
Molecular weight
32.57 kDa
pI
5.71
function
rRNA maturation
product
rRNA adenine dimethyltransferase
essential
no
ec
2.1.1.-
synonyms
ksgA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0030 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
50,640 → 51,518
Phenotypes of a mutant
defective in swarming motility (reduced rate of colony expansion) [pubmed|35638827]
The protein
Catalyzed reaction/ biological activity
adenosine1518/adenosine1519 in 16S rRNA + 4 S-adenosyl-L-methionine --> 4 H+ + N6-dimethyladenosine1518/N6-dimethyladenosine1519 in 16S rRNA + 4 S-adenosyl-L-homocysteine (according to UniProt)
Protein family
[wiki|Methyltransferase superfamily] (according to UniProt)
[wiki|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)
Structure
[PDB|6IFT] (complexed with S-adenosylmethionine) [Pubmed|30624914]
[PDB|6IFS] [Pubmed|30624914]
[PDB|6IFV] (C-terminal truncation) [Pubmed|30624914]
[PDB|6IFW] (Chimeric protein) [Pubmed|30624914]
[AF|P37468]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|5BA7AA691CFE4635DEFBD0B8B820F487B58A5DB7|rnmV]-[gene|FF329359CBE52C75A39237E8A6BB56F6AB321AFB|ksgA]
description
[Pubmed|11233981]
Biological materials
Mutant
MGNA-B907 (ksgA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1906 NBRP B. subtilis, Japan]
BKE00420 (Δ[gene|FF329359CBE52C75A39237E8A6BB56F6AB321AFB|ksgA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTTATTCATTCTGTTCCTC, downstream forward: _UP4_TAAAAGGGCTTTTTGTTTTG
BKK00420 (Δ[gene|FF329359CBE52C75A39237E8A6BB56F6AB321AFB|ksgA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTTATTCATTCTGTTCCTC, downstream forward: _UP4_TAAAAGGGCTTTTTGTTTTG
References
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Time of last update: 2024-12-21 11:07:45
Author of last update: Jstuelk