ansA
168
L-asparaginase
locus
BSU_23580
Molecular weight
36.30 kDa
pI
4.45
function
[metabolite|asparagine] degradation
product
L-asparaginase
essential
no
ec
3.5.1.1
synonyms
ansA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0252 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,455,819 → 2,456,808
The protein
Catalyzed reaction/ biological activity
H2O + L-[metabolite|asparagine] --> L-[metabolite|aspartate] + NH4+ (according to UniProt)
Protein family
asparaginase 1 family (with [protein|B5301B346FBAAE2D7C37D561FE1DCFDDFAC90D40|ansZ], according to UniProt)
[wiki|Domains]
Asparaginase/glutaminase domain (aa 2-322) (according to UniProt)
Structure
[PDB|5OT0] (from Thermococcus kodakarensis, 43% identity) [pubmed|29095161]
[AF|P26900]
Paralogous protein(s)
[protein|B5301B346FBAAE2D7C37D561FE1DCFDDFAC90D40|ansZ]
Expression and Regulation
Operons
genes
[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]
description
[Pubmed|1711029]
regulation
expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
[protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]: repression, [Pubmed|11914346], in [regulon|protein:146947C974D62BCB282BA3F5B924B35695D75886|ansR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1711029], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]-[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]-[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]
description
[Pubmed|22383849]
regulation
[protein|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]: expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
expression
expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
[protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]: transcription repression, [pubmed|11914346], in [regulon|protein:146947C974D62BCB282BA3F5B924B35695D75886|ansR regulon]
Biological materials
Mutant
GP1153 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::''ermC'') available in [wiki|Jörg Stülke]'s lab
GP4256 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::''cat'') available in [wiki|Jörg Stülke]'s lab
BKE23580 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCATGCACCTCTTCAC, downstream forward: _UP4_TAAAATGTAAAAACGATAAC
BKK23580 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCATGCACCTCTTCAC, downstream forward: _UP4_TAAAATGTAAAAACGATAAC
lacZ fusion
pGP872 (in [wiki|pAC5]), available in [wiki|Stülke] lab
References
Page visits: 9188
Time of last update: 2024-11-09 15:06:11
Author of last update: Jstuelk