leuS
168
Leu-tRNA synthetase
locus
BSU_30320
Molecular weight
91.35 kDa
pI
4.86
function
translation
product
leucyl-tRNA synthetase
essential
yes
ec
6.1.1.4
synonyms
leuS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0495 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,102,629 → 3,105,043
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
ATP + L-leucine + tRNALeu --> AMP + diphosphate + L-leucyl-tRNALeu (according to UniProt)
Protein family
[wiki|Class-I aminoacyl-tRNA synthetase family] (according to UniProt)
Structure
[PDB|1H3N] (from Thermus thermophilus, 46.4% identity) [pubmed|10811626]
[PDB|2AJG] (from ''Escherichia coli'', 39% identity, 56% similarity, 22% coverage) [Pubmed|16277600]
[AF|P36430]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|FEC89C6F5A825D925D730965ACBAA06EC5CC4E44|leuS]
description
[Pubmed|1317842]
regulation
induced by leucine limitation ([protein|search|T-box]) [Pubmed|1317842]
regulatory mechanism
T-box: RNA switch, via uncharged tRNA-Leu binding to [wiki|RNA switch], in [regulon|other_regulator:T-box|T-box]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|1317842], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
References
Reviews
Original Publications
Page visits: 2613
Time of last update: 2025-04-15 01:50:58
Author of last update: Jstuelk