cimH
168
transporter for citrate (proton symport)
locus
BSU_38770
Molecular weight
48.18 kDa
pI
6.55
function
citrate uptake
product
transporter for citrate (proton symport)
essential
no
synonyms
cimH, yxkJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3493 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,979,753 → 3,981,105
The protein
Catalyzed reaction/ biological activity
uptake of citrate (symport with protons) [Pubmed|11566984]
Protein family
sodium:citrate (SCF) symporter family (together with [protein|F8F62635AA87E4E3A255D6F8FC621EF40954B520|maeN], according to UniProt)
Structure
[PDB|5X9R] (from Klebsiella pneumoniae, 34% identity) [pubmed|28566738]
[AF|P94363]
Paralogous protein(s)
[protein|F8F62635AA87E4E3A255D6F8FC621EF40954B520|maeN]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|FEC0EDE9663FE62B2A1A4FA5B16B41048DDD723B|cimH]
description
[Pubmed|21815947]
regulation
repressed by glucose ([protein|search|CcpA]) [Pubmed|10666464]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
additional information
the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
Biological materials
Mutant
MGNA-B744 (yxkJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1743 NBRP B. subtilis, Japan]
BKE38770 (Δ[gene|FEC0EDE9663FE62B2A1A4FA5B16B41048DDD723B|cimH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGAAAAACCTCCCCTAT, downstream forward: _UP4_TGATCCCCGCAAGCAAGTTC
BKK38770 (Δ[gene|FEC0EDE9663FE62B2A1A4FA5B16B41048DDD723B|cimH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGAAAAACCTCCCCTAT, downstream forward: _UP4_TGATCCCCGCAAGCAAGTTC
GP4216 ([gene|FEC0EDE9663FE62B2A1A4FA5B16B41048DDD723B|cimH]::kan), available in Jörg Stülke's lab
References
Reviews
Original Publications
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Time of last update: 2024-12-18 12:14:28
Author of last update: Christoph.elfmann