yisZ
168
adenylylsulfate kinase
locus
BSU_10910
Molecular weight
22.16 kDa
pI
7.21
function
sulfate reduction and activation
product
adenylylsulfate kinase
essential
no
ec
2.7.1.25
synonyms
yisZ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0529 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,169,877 → 1,170,476
The protein
Catalyzed reaction/ biological activity
adenosine 5'-phosphosulfate + ATP --> 3'-phosphoadenylyl sulfate + ADP + H+ (according to UniProt)
Protein family
APS kinase family (with [protein|3C71659E4868744A9301C26608EABF7B98217DCF|cysC], according to UniProt)
Structure
[PDB|3CR8] (from ''Escherichia coli'', 38% identity, 55% similarity) [Pubmed|19770499]
[AF|O06735]
Expression and Regulation
Operons
genes
[gene|E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E|yitB]-[gene|EBBA4432FF83B6F203C694E04111D45290BE157A|yitA]-[gene|FE2B31E5EBE6C4BA4FC4AAC485A00C97315E6AAB|yisZ]
description
[Pubmed|22383849]
regulation
expressed during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|15699190,15383836]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,15383836], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
Biological materials
Mutant
MGNA-A499 (yisZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/499 NBRP B. subtilis, Japan]
BKE10910 (Δ[gene|FE2B31E5EBE6C4BA4FC4AAC485A00C97315E6AAB|yisZ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCAAATGATGTTCGGATTGT, downstream forward: _UP4_TAAAAAACCCGATCAATGTC
BKK10910 (Δ[gene|FE2B31E5EBE6C4BA4FC4AAC485A00C97315E6AAB|yisZ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCAAATGATGTTCGGATTGT, downstream forward: _UP4_TAAAAAACCCGATCAATGTC
References
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Time of last update: 2024-12-31 00:41:31
Author of last update: Melvin.boenninger