ccpC
168
transcriptional repressor of [gene|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ] and [gene|E15E9F88B0E03FE8834C646369D2F4B4E713EF41|citB]
locus
BSU_14140
Molecular weight
33.76 kDa
pI
5.93
function
regulation of tricarboxylic acid branch of the TCA cycle
product
transcriptional repressor ([wiki|LysR family])
essential
no
synonyms
ccpC, ykuM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0583 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,486,045 → 1,486,926
The protein
Protein family
[wiki|LysR family] (according to UniProt)
[wiki|Domains]
[wiki|HTH lysR-type domain] (aa 1-58) (according to UniProt)
Structure
[PDB|5Z72] (from B. amyloliquefaciens, 96% identity)
[AF|O34827]
Expression and Regulation
Operons
genes
[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]
description
[Pubmed|16395550]
regulation
repressed in the presence of glucose and glutamate ([protein|search|CcpC]) [Pubmed|11985717]
regulatory mechanism
[protein|FE15A41A58A8177280817CA3825764C39185021A|ccpC]: repression, (molecular inducer: citrate) [Pubmed|11985717], in [regulon|protein:FE15A41A58A8177280817CA3825764C39185021A|ccpC regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|16395550], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]-[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]-[gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]-[gene|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]-[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]
description
[Pubmed|16395550]
regulation
expressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, by [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]~P [Pubmed|18840696,14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|16395550,12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
Biological materials
Mutant
GP3593 (Δ[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]::''phleo''), available in [wiki|Jörg Stülke]'s lab
MGNA-A774 (ykuM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/774 NBRP B. subtilis, Japan]
GP1442 (''aphA3'') , available in [wiki|Jörg Stülke]'s lab
BKE14140 (Δ[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGTCCTCCTTTTTGT, downstream forward: _UP4_TAAAGACAGCGAGGTGCTGT
BKK14140 (Δ[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGTCCTCCTTTTTGT, downstream forward: _UP4_TAAAGACAGCGAGGTGCTGT
GP2800 (''Δ''''[gene|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]-[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]]''::''phleo''), available in [wiki|Jörg Stülke]'s lab
GP3593 (''Δ''''[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]''::''phleo''), available in [wiki|Jörg Stülke]'s lab
Expression vectors
for expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP706, available in [wiki|Jörg Stülke]'s lab
pGP3929 (C-terminal Strep-tag, purification from E. coli, in [wiki|pGP574]), available in [wiki|Jörg Stülke]'s lab
pGP3936 (N-terminal Strep-tag, purification from E. coli, in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP707 (in [wiki|pAC5]), available in [wiki|Stülke] lab, pGP711 (in [wiki|pAC6]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
References
Page visits: 7444
Time of last update: 2024-11-20 17:34:20
Author of last update: Dennis.wicke