rhaA
168
L-rhamnose isomerase
locus
BSU_31180
Molecular weight
48.48 kDa
pI
5.35
function
rhamnose utilization
product
L-rhamnose isomerase
essential
no
ec
5.3.1.14
synonyms
rhaA, yulE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4806 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,197,933 → 3,199,207
The protein
Catalyzed reaction/ biological activity
L-rhamnose = L-rhamnulose [Pubmed|26712933]
Protein family
rhamnose isomerase family (single member, according to UniProt)
Structure
[PDB|3P14] (from B. halodurans, 78% identity) [pubmed|24925069]
[AF|O05264]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|C32AE30BF7931D6171E9DE75C67504F81DD0F227|rhaEW]-[gene|F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR]-[gene|693040A5BA7A25AD485B7B28DBD6E8AAC77450F7|rhaB]-[gene|294C0CC675088B2A2EEFC26FAF1A628002B82C59|rhaM]-[gene|FDDC16D57752D81299CDD426257C1CD49890C89C|rhaA]
description
[Pubmed|26712933,22383849]
regulation
expression during spore [wiki|germination] is increased under conditions of osmotic stress [Pubmed|27766092]
regulatory mechanism
[protein|F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR]: repression, [Pubmed|26712933], in [regulon|protein:F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|26712933], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|26712933], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR]-[gene|693040A5BA7A25AD485B7B28DBD6E8AAC77450F7|rhaB]-[gene|294C0CC675088B2A2EEFC26FAF1A628002B82C59|rhaM]-[gene|FDDC16D57752D81299CDD426257C1CD49890C89C|rhaA]
description
[Pubmed|22383849]
regulation
expression during spore [wiki|germination] is increased under conditions of osmotic stress [Pubmed|27766092]
Biological materials
Mutant
MGNA-B544 (yulE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1543 NBRP B. subtilis, Japan]
BKE31180 (Δ[gene|FDDC16D57752D81299CDD426257C1CD49890C89C|rhaA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACGGCTCTCTCCCTTTA, downstream forward: _UP4_TAATAAAAAATCCGCCGCGG
BKK31180 (Δ[gene|FDDC16D57752D81299CDD426257C1CD49890C89C|rhaA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACGGCTCTCTCCCTTTA, downstream forward: _UP4_TAATAAAAAATCCGCCGCGG
References
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Time of last update: 2024-11-07 22:58:42
Author of last update: Melvin.boenninger