pghL
168
phage-derived gamma polyglutamic acid hydrolase, sporulation protein
locus
BSU_17820
Molecular weight
28.07 kDa
pI
6.92
function
polyglutamic acid degradation
product
phage-derived gamma polyglutamic acid hydrolase
essential
no
synonyms
pghL, yndL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4195 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,915,221 → 1,915,979
The protein
Catalyzed reaction/ biological activity
hydrolysis of polyglutamic acid [Pubmed|26158264]
Protein family
[wiki|UPF0714 family] (according to UniProt)
[wiki|Domains]
DUF867 [http://pfam.xfam.org/family/PF05908 x], [[gamma-PGA hydrolase domain]] [Pubmed|26158264]
Structure
[PDB|5ONJ] (complex with poly-γ-glutamate) [pubmed|30387270]
[PDB|3A9L] (from B. subtilis phage NIT1, 36% identity) [pubmed|22105902]
[PDB|6HRI] (native)
[PDB|6HRJ] (apo-form)
[AF|O31815]
Paralogous protein(s)
[protein|8F9DCD77660706E4D25CD7875C014F6A2B5D8123|pghZ], [protein|BA7D1D112E934B68073DFC1F76248ED6157240E3|pghB], [protein|DF95DED9A6E2160C151D7664C951B198EEA225E8|pghC]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|FD862CE6C4A37B17714F8F8687B3266D43EF5F63|pghL]
description
[Pubmed|16385044]
regulation
expressed during [wiki|sporulation] in the mother cell[Pubmed|16385044]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,16385044], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
additional information
An [antisense RNA], '[protein|search|surA]', is predicted for '[protein|search|pghL]' [PubMed|20525796]
Biological materials
Mutant
MGNA-B113 (yndL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1112 NBRP B. subtilis, Japan]
BKE17820 (Δ[gene|FD862CE6C4A37B17714F8F8687B3266D43EF5F63|pghL]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTACTCCGCCTCTAAGT, downstream forward: _UP4_TGAAAAATAGGAGGCGATAA
BKK17820 (Δ[gene|FD862CE6C4A37B17714F8F8687B3266D43EF5F63|pghL]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTACTCCGCCTCTAAGT, downstream forward: _UP4_TGAAAAATAGGAGGCGATAA
References
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Time of last update: 2024-10-12 10:27:26
Author of last update: Melvin.boenninger