fenA
168
magnesium-dependent 5'->3' flap endonuclease, major contributor to the removal of RNA from an Okazaki fragment, protection of developing and dormant spores against UV DNA damage
locus
BSU_22010
Molecular weight
32.79 kDa
pI
5.22
function
removal of RNA from Okazaki fragments
product
5′-3′ flap endonuclease
essential
no
synonyms
exoR, ypcP, exoA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0258 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,310,995 → 2,311,885
Phenotypes of a mutant
a [gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA] [gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|fenA] double mutant is not viable (synthetic lethality) [pubmed|17114254]
a [gene|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC] [gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|fenA] double mutant is not viable at 25°C [pubmed|30670546]
The protein
Catalyzed reaction/ biological activity
removal of RNA from Okazaki fragments [pubmed|37146086,30670546]
magnesium-dependent exonuclease, shows highest binding affinity to 5′ overhangs in dsDNA [pubmed|31251806]
[wiki|Domains]
5'-3' exonuclease domain (aa 175-262) (according to UniProt)
[wiki|Cofactors]
Mg2+ [pubmed|31251806]
Structure
[PDB|1BGX] (Taq polymerase, 36% identity) [pubmed|9770525]
[AF|P54161]
Paralogous protein(s)
[protein|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA], one of the two proteins has to be present for viability [Pubmed|17114254]
[wiki|Localization]
recruited to replication forks after induction of DNA damage [pubmed|31251806]
recruited to the replication forks [pubmed|39195267]
Expression and Regulation
Operons
genes
[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|fenA]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-B868 (exoA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1867 NBRP B. subtilis, Japan]
BKE22010 (Δ[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|fenA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAATAACTCCCTTTTC, downstream forward: _UP4_GCTAGAGAGATCGTTTAGAT
BKK22010 (Δ[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|fenA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAATAACTCCCTTTTC, downstream forward: _UP4_GCTAGAGAGATCGTTTAGAT
References
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Time of last update: 2024-12-20 20:38:18
Author of last update: Jstuelk