fenA

fenA
168

magnesium-dependent 5'->3' flap endonuclease, major contributor to the removal of RNA from an Okazaki fragment, protection of developing and dormant spores against UV DNA damage

Locus
BSU_22010
Molecular weight
32.79 kDa
Isoelectric point
5.22
Protein length
296 aaSequenceBlast
Gene length
888 bpSequenceBlast
Function
removal of RNA from Okazaki fragments
Product
5′-3′ flap endonuclease
Essential
no
Synonyms
exoR, ypcP, exoA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0258 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,310,995 → 2,311,885
Phenotypes of a mutant
a polA fenA double mutant is not viable (synthetic lethality) PubMed
a rnhC fenA double mutant is not viable at 25°C PubMed
The protein
Catalyzed reaction/ biological activity
removal of RNA from Okazaki fragments PubMed
magnesium-dependent exonuclease, shows highest binding affinity to 5′ overhangs in dsDNA PubMed
Domains
5'-3' exonuclease domain (aa 175-262) (according to UniProt)
Cofactors
Mg2+ PubMed
Structure
1BGX (PDB) (Taq polymerase, 36% identity) PubMed
P54161 (AlphaFold)
Paralogous protein(s)
PolA, one of the two proteins has to be present for viability PubMed
Localization
recruited to replication forks after induction of DNA damage PubMed
recruited to the replication forks PubMed
Expression and Regulation
Operons
Genes
fenA
Description
PubMed
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fenA

2025-02-14 05:01:55

Jstuelk

88

491a128243b0ca52c514cd6984cca40c6784ee4d

1F5E708A006B52193A837C0B07F667DEF17E9A78

Biological materials
Mutant
MGNA-B868 (exoA::erm), available at the NBRP B. subtilis, Japan
BKE22010 (ΔfenA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAAATAACTCCCTTTTC,  downstream forward: _UP4_GCTAGAGAGATCGTTTAGAT
BKK22010 (ΔfenA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAAATAACTCCCTTTTC,  downstream forward: _UP4_GCTAGAGAGATCGTTTAGAT
References
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Time of last update: 2025-04-11 05:11:03

Author of last update: Jstuelk