plsC
168
acyl-ACP:1-acylglycerolphosphate acyltransferase
locus
BSU_09540
Molecular weight
21.78 kDa
pI
9.98
function
biosynthesis of phospholipids
product
acyl-ACP:1-acylglycerolphosphate acyltransferase
essential
yes
ec
2.3.1.51
synonyms
plsC, yhdO
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0204 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,031,395 → 1,031,994
Phenotypes of a mutant
essential [Pubmed|12682299]
specific point mutations affecting [protein|FC05D5FC95CEDC4B4A04040C86170D185528B9FF|plsC] (R48P, A61V, or P110S) facilitate the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate, especially in combination with [protein|5ACE71EBBDCEB0C534A91DE303FD8069BEFDD6A1|yfkC]-R42W [pubmed|33481774]
The protein
Catalyzed reaction/ biological activity
Acyl-[protein|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|acpA] + 1-acyl-sn-glycerol 3-phosphate --> [protein|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|acpA] + 1,2-diacyl-sn-glycerol 3-phosphate (according to UniProt)
Protein family
1-acyl-sn-glycerol-3-phosphate acyltransferase family (single member, according to UniProt)
Structure
[PDB|5KYM] (from Thermotoga maritima) [pubmed|28714993]
[AF|O07584]
[wiki|Localization]
cell membrane at the septum [Pubmed|15743965]
Expression and Regulation
Operons
genes
[gene|4DF9B105ABA64AFD5106EE75FCC9EFAB52BEFC83|yhdN]-[gene|FC05D5FC95CEDC4B4A04040C86170D185528B9FF|plsC]
description
[Pubmed|10220166]
regulation
induced by stress ([protein|search|SigB]) [Pubmed|10220166,15805528]
regulatory mechanism
[protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]: repression, [Pubmed|12737802], in [regulon|protein:FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR regulon]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|10220166,15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
MGNA-B483 (yhdO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1482 NBRP B. subtilis, Japan]
GP3131 ([gene|5ACE71EBBDCEB0C534A91DE303FD8069BEFDD6A1|yfkC]-R42W kan [gene|FC05D5FC95CEDC4B4A04040C86170D185528B9FF|plsC]-L48P phleo) , available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP3683 (''spc'', based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
GP3674 (based on [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-12-21 15:03:02
Author of last update: Rica