asnS
168
asparagyl-tRNA synthetase
locus
BSU_22360
Molecular weight
48.92 kDa
pI
5.12
function
translation
product
asparagyl-tRNA synthetase
essential
no
ec
6.1.1.22
synonyms
asnS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0017 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,346,224 → 2,347,516
Phenotypes of a mutant
essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]
The protein
Catalyzed reaction/ biological activity
ATP + L-asparagine + tRNAAsn --> AMP + diphosphate + H+ + L-asparaginyl-tRNAAsn (according to UniProt)
Protein family
[wiki|Class-II aminoacyl-tRNA synthetase family] (according to UniProt)
Structure
[PDB|1X56] (from ''Pyrococcus horikoshii'', 42% identity) [Pubmed|16753178]
[AF|P39772]
Expression and Regulation
Operons
genes
[gene|FBA7EAA70F5C9E76463B0C5F85D65D4314FFD40D|asnS]-[gene|C9FEE07601AD28B2651EB881A1D9B34D5082237C|dnaD]-[gene|C352560155F5B2E951B5059794D7B319C1A3AF2C|nth]-[gene|4B2A124B726393544A47791E5A5681F7B766A588|ypoC]
description
[Pubmed|22383849]
Biological materials
Mutant
GP2934 (10 bp deletion at bp 1013) (no resistance) available in [wiki|Jörg Stülke]'s lab
BKE22360 (Δ[gene|FBA7EAA70F5C9E76463B0C5F85D65D4314FFD40D|asnS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACATATTTCCCTCCAAAA, downstream forward: _UP4_TAATACATTCAAATGAAACA
BKK22360 (Δ[gene|FBA7EAA70F5C9E76463B0C5F85D65D4314FFD40D|asnS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACATATTTCCCTCCAAAA, downstream forward: _UP4_TAATACATTCAAATGAAACA
References
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Time of last update: 2024-12-19 20:08:21
Author of last update: Melvin.boenninger