cysL
168
transcriptional activator of the [gene|5A99F57DF0DDF5A129C3702E97621232A8173C22|cysJ]-[gene|A4C9E4D1163B703A917BFCF97A5F575B19EDDD8D|cysI] operon
locus
BSU_37650
Molecular weight
34.04 kDa
pI
6.96
function
regulation of cysteine biosynthesis
product
transcriptional regulator ([wiki|LysR family])
essential
no
synonyms
cysL, ywfK, ipa-89d
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0583 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,864,309 → 3,865,208
Phenotypes of a mutant
unable to grow with sulfate or sulfite as the only sulfur source [Pubmed|12169591]
defective in [wiki|biofilm formation] [pubmed|30718304]
The protein
Protein family
[wiki|LysR family] (according to UniProt)
[wiki|Domains]
[wiki|HTH lysR-type domain] (aa 1-58) (according to UniProt)
Structure
[PDB|5Y2V] (from Synechocystis sp., 31% identity) [pubmed|29279392]
[AF|P39647]
Expression and Regulation
Operons
genes
[gene|FB37E9B7F7727915ADB18E52FD07A8773C7B30FC|cysL]
description
[Pubmed|12169591]
regulation
repressed by sulfate, sulfite, and thiosulfate ([protein|search|CysL]) [Pubmed|12169591]
regulatory mechanism
[protein|FB37E9B7F7727915ADB18E52FD07A8773C7B30FC|cysL]: auto-repression, [Pubmed|12169591], in [regulon|protein:FB37E9B7F7727915ADB18E52FD07A8773C7B30FC|cysL regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12169591], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B668 (ywfK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1667 NBRP B. subtilis, Japan]
BKE37650 (Δ[gene|FB37E9B7F7727915ADB18E52FD07A8773C7B30FC|cysL]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAACCCCACCCCTGTCA, downstream forward: _UP4_TGATATAATAGTTTTCGTTC
BKK37650 (Δ[gene|FB37E9B7F7727915ADB18E52FD07A8773C7B30FC|cysL]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAACCCCACCCCTGTCA, downstream forward: _UP4_TGATATAATAGTTTTCGTTC
References
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Time of last update: 2024-12-24 01:46:32
Author of last update: Melvin.boenninger